CAB39 Rabbit Polyclonal Antibody
Catalog# ER60546
CAB39 Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
CAB39 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CAB39 aa 1-341.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 40 kDa
Positive Control
MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000-1:10,000
Target
Function
Calcium-binding protein 39 is a protein that in humans is encoded by the CAB39 gene. The protein encoded by this gene associates with STK11 (Serine/Threonine Kinase 11) and STRAD (STE20-Related ADaptor protein). CAB39 enhances formation of STK11/STRAD complexes and stimulates STK11 catalytic activity. CAB39 may function as a scaffolding component of the STK11/STRAD complex and regulates STK11 activity and cellular localization.
Background References
1. Gao D et al. CAB39 promotes cisplatin resistance in bladder cancer via the LKB1-AMPK-LC3 pathway. Free Radic Biol Med. 2023 Nov
2. Ferdaus MZ et al. Kinase Scaffold Cab39 Is Necessary for Phospho-Activation of the Thiazide-Sensitive NCC. Hypertension. 2024 Apr
Subcellular Location
Cytoplasm.
Synonyms
Cab39 antibody
CAB39_HUMAN antibody
Calcium binding protein 39 antibody
Calcium-binding protein 39 antibody
CGI 66 antibody
CGI-66 antibody
CGI66 antibody
MO25 alpha antibody
MO25 antibody
Mouse protein 25 alpha antibody
ExpandImages
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Western blot analysis of CAB39 on different lysates with Rabbit anti-CAB39 antibody (ER60546) at 1/5,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER60546) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CAB39 on different lysates with Rabbit anti-CAB39 antibody (ER60546) at 1/10,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse testis tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER60546) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CAB39 Recombinant Rabbit Monoclonal Antibody [PSH15-16] - BSA and Azide free
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
