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alpha 2a Adrenergic Receptor Rabbit Polyclonal Antibody

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Catalog# ER62703

alpha 2a Adrenergic Receptor Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

alpha 2a Adrenergic Receptor Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human ADRA2A aa 1-48.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 51 kDa

Positive Control

SH-SY5Y cell lysate, PC-12 cell lysate, Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat hippocampus tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000

Target

Function

The alpha-2A adrenergic receptor (α2A adrenoceptor), also known as ADRA2A, is an α2-adrenergic receptor, and also denotes the human gene encoding it. α2-adrenergic receptors include 3 highly homologous subtypes: α2A, α2B, and α2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mice revealed that both the α2A and α2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the α2A subtype inhibited transmitter release at high stimulation frequencies, whereas the α2C subtype modulated neurotransmission at lower levels of nerve activity.

Background References

1. Li C et al. Enterococcus-derived tyramine hijacks alpha(2A)-adrenergic receptor in intestinal stem cells to exacerbate colitis. Cell Host Microbe. 2024 Jun

2. Fink EA et al. Structure-based discovery of nonopioid analgesics acting through the alpha(2A)-adrenergic receptor. Science. 2022 Sep

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P08913 Human

Synonyms

ADA2A_HUMAN antibody

ADRA2 antibody

ADRA2A antibody

ADRA2R antibody

ADRAR antibody

Adrenergic alpha 2A receptor antibody

Alpha 2 adrenergic receptor subtype C10 antibody

Alpha 2A adrenergic receptor antibody

Alpha 2AAR subtype C10 antibody

Alpha-2 adrenergic receptor subtype C10 antibody

Expand

Images

  • Western blot analysis of alpha 2a Adrenergic Receptor on different lysates with Rabbit anti-alpha 2a Adrenergic Receptor antibody (<a href="/products/ER62703" style="font-weight: bold;text-decoration: underline;">ER62703</a>) at 1/10,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (no heat)<br />Lane 2: PC-12 cell lysate<br />Lane 3: Mouse hippocampus tissue lysate (no heat)<br />Lane 4: Mouse brain tissue lysate (no heat)<br />Lane 5: Rat hippocampus tissue lysate<br />Lane 6: Rat brain tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER62703" style="font-weight: bold;text-decoration: underline;">ER62703</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of alpha 2a Adrenergic Receptor on different lysates with Rabbit anti-alpha 2a Adrenergic Receptor antibody (ER62703) at 1/10,000 dilution.

    Lane 1: SH-SY5Y cell lysate (no heat)
    Lane 2: PC-12 cell lysate
    Lane 3: Mouse hippocampus tissue lysate (no heat)
    Lane 4: Mouse brain tissue lysate (no heat)
    Lane 5: Rat hippocampus tissue lysate
    Lane 6: Rat brain tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 55 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER62703) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation