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MAP4K1 / HPK1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER63713

MAP4K1 / HPK1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MAP4K1 / HPK1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human MAP4K1 aa 301-500.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 91 kDa

Positive Control

Raji cell lysate, Daudi cell lysate, human spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:200

Target

Function

catalytic activity:ATP + a protein = ADP + a phosphoprotein. cofactor:Magnesium. May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain. subunit:Interacts with MAP3K1. tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.

Background References

1. Sun JM et al. Glioblastoma cellular MAP4K1 facilitates tumor growth and disrupts T effector cell infiltration. Life Sci Alliance. 2023 Sep

2. He TS et al. The Kinase MAP4K1 Inhibits Cytosolic RNA-Induced Antiviral Signaling by Promoting Proteasomal Degradation of TBK1/IKKepsilon. Microbiol Spectr. 2021 Dec

Subcellular Location

Cytoplasm, membrane.

UNIPROT

SwissProt: Q92918 Human

Synonyms

Hematopoietic progenitor kinase 1 antibody

Hematopoietic progenitor kinase antibody

HPK1 antibody

Human Hematopoietic Progenitor Kinase antibody

M4K1_HUMAN antibody

Map4k1 antibody

MAPK/ERK kinase kinase kinase 1 antibody

MEK kinase kinase 1 antibody

MEKKK 1 antibody

Mitogen activated protein kinase kinase kinase kinase 1 antibody

Expand

Images

  • Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody (<a href="/products/ER63713" style="font-weight: bold;text-decoration: underline;">ER63713</a>) at 1/2,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: Daudi cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 91 kDa<br />Observed band size: 97 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER63713" style="font-weight: bold;text-decoration: underline;">ER63713</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody (ER63713) at 1/2,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: Daudi cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 91 kDa
    Observed band size: 97 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63713) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (<a href="/products/ER63713" style="font-weight: bold;text-decoration: underline;">ER63713</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER63713" style="font-weight: bold;text-decoration: underline;">ER63713</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (ER63713) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER63713) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (<a href="/products/ER63713" style="font-weight: bold;text-decoration: underline;">ER63713</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER63713" style="font-weight: bold;text-decoration: underline;">ER63713</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (ER63713) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER63713) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24]

Application: WB,IHC-P,IP

Reactivity: Human

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MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24]

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MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24] - BSA and Azide free

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