Hexokinase Type III / HK3 Rabbit Polyclonal Antibody
Catalog# ER63723
Hexokinase Type III / HK3 Rabbit Polyclonal Antibody
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WB
-
Mouse
-
unconjugated
Overview
Product Name
Hexokinase Type III / HK3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 100 kDa
Positive Control
Mouse spleen tissue lysate, Mouse colon tissue lysate.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Hexokinase III, also known as hexokinase C, is an enzyme which in humans is encoded by the Hk3 gene on chromosome 5. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Similar to hexokinases I and II, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Background References
1. Li S et al. HK3 stimulates immune cell infiltration to promote glioma deterioration. Cancer Cell Int. 2023 Oct
2. Zhang W et al. Hexokinase HK3-mediated O-GlcNAcylation of EP300: a key regulator of PD-L1 expression and immune evasion in ccRCC. Cell Death Dis. 2024 Aug
Subcellular Location
Mitochondrion.
UNIPROT
Synonyms
ATP:D hexose 6 phosphotransferase antibody
Hexokinase type III antibody
Hexokinase-3 antibody
Hexokinase3 antibody
HK III antibody
Hk3 antibody
HKIII antibody
HXK3 antibody
HXK3_HUMAN antibody
Images
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Western blot analysis of Hexokinase Type III / HK3 on different lysates with Rabbit anti-Hexokinase Type III / HK3 antibody (ER63723) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse colon tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63723) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"