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Oxytocin-neurophysin 1 / OXT Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER64173

Oxytocin-neurophysin 1 / OXT Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Oxytocin-neurophysin 1 / OXT Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human OXT aa 1-125.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 13 kDa

Positive Control

Mouse pituitary tissue lysates, mouse pituitary tissue, rat pituitary tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:8,000

Target

Function

This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

Background References

暂无

Subcellular Location

Secreted.

UNIPROT

SwissProt: P35454 Mouse

Entrez Gene: 25504 Rat

Synonyms

MGC126890 antibody

MGC126892 antibody

Neurophysin 1 antibody

Neurophysin I antibody

Ocytocin antibody

OT antibody

OT NPI antibody

OXT antibody

Oxytocin antibody

Oxytocin Neurophysin preproprotein antibody

Expand

Images

  • Western blot analysis of Oxytocin-neurophysin 1 / OXT on mouse pituitary tissue lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/ER64173" style="font-weight: bold;text-decoration: underline;">ER64173</a>) at 1/2,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 13 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER64173" style="font-weight: bold;text-decoration: underline;">ER64173</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Oxytocin-neurophysin 1 / OXT on mouse pituitary tissue lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (ER64173) at 1/2,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 13 kDa
    Observed band size: 14 kDa

    Exposure time: 3 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64173) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/ER64173" style="font-weight: bold;text-decoration: underline;">ER64173</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER64173" style="font-weight: bold;text-decoration: underline;">ER64173</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (ER64173) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER64173) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/ER64173" style="font-weight: bold;text-decoration: underline;">ER64173</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER64173" style="font-weight: bold;text-decoration: underline;">ER64173</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (ER64173) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER64173) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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