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PJA2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER64613

PJA2 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PJA2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human PJA2 aa 31-80.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 78 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, U-87 MG cell lysate.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Has E2-dependent E3 ubiquitin-protein ligase activity. pathway:Protein modification; protein ubiquitination. Contains 1 RING-type zinc finger. subcellular location:Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses. subunit:Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2.

Background References

1. Schiefer S et al. Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by PJA2. Nat Commun. 2024 May

2. Liu B et al. ZC3H13 promotes autophagy in bladder cancer through m6A methylation modification of PJA2 and ubiquitination of KSR1. Hum Cell. 2024 Nov

Subcellular Location

Cytoplasm, Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Synapse, Postsynaptic density, cytoskeleton, microtubule organizing center, centrosome.

UNIPROT

SwissProt: O43164 Human

Synonyms

E3 ubiquitin protein ligase Praja2 antibody

E3 ubiquitin-protein ligase Praja-2 antibody

KIAA0438 antibody

Neurodap 1 antibody

Neurodap1 antibody

PJA 2 antibody

PJA2 antibody

PJA2_HUMAN antibody

Praja 2 antibody

Praja 2 RING H2 motif containing antibody

Expand

Images

  • Western blot analysis of PJA2 on different lysates with Rabbit anti-PJA2 antibody (<a href="/products/ER64613" style="font-weight: bold;text-decoration: underline;">ER64613</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: U-87 MG cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER64613" style="font-weight: bold;text-decoration: underline;">ER64613</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PJA2 on different lysates with Rabbit anti-PJA2 antibody (ER64613) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: U-87 MG cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 150 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64613) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"