SRPK1 Rabbit Polyclonal Antibody
Catalog# ER65028
SRPK1 Rabbit Polyclonal Antibody
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WB
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Human
-
unconjugated
Overview
Product Name
SRPK1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human SRPK1 aa 251-500.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 74 kDa
Positive Control
Jurkat cell lysate, Caco-2 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, BxPC-3 cell lysate.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:10,000
Target
Function
Serine/arginine-Rich Splicing Factor (SRSF) protein kinase-1 SRPK1 is an enzyme that in humans is encoded by the SRPK1 gene. This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined. SRPK1 enables angiogenesis, which is regulated by VEGF, which either initiates or inhibits vessel formation depending on alternative splicing.
Background References
1. Duggan WP et al. Serine-Arginine Protein Kinase 1 (SRPK1): a systematic review of its multimodal role in oncogenesis. Mol Cell Biochem. 2022 Oct
2. Guo W et al. SRPK1 promotes sepsis-induced acute lung injury via regulating PI3K/AKT/FOXO3 signaling. Immunopharmacol Immunotoxicol. 2023 Apr
Subcellular Location
Cytoplasm, Nucleus, nucleoplasm, Nucleus speckle, Chromosome.
UNIPROT
Synonyms
Serine/arginine rich protein specific kinase 1 antibody
Serine/arginine rich splicing factor kinase 1 antibody
Serine/arginine-rich protein-specific kinase 1 antibody
Serine/threonine protein kinase SRPK1 antibody
Serine/threonine-protein kinase SRPK1 antibody
SFRS protein kinase 1 antibody
SFRSK1 antibody
SR protein kinase 1 antibody
SR protein specific kinase 1 antibody
SR-protein-specific kinase 1 antibody
ExpandImages
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Western blot analysis of SRPK1 on different lysates with Rabbit anti-SRPK1 antibody (ER65028) at 1/10,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: BxPC-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 100 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65028) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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