SOCS3 Rabbit Polyclonal Antibody (ER65765)
Catalog# ER65765
Rabbit Polyclonal to SOCS3
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: PC-3M cell lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
SOCS3 Rabbit Polyclonal Antibody (ER65765)
Immunogen
Recombinant protein within human
Host
Rabbit
Positive Control
PC-3M cell lysate, mouse spleen tissue lysate, mouse colon tissue lysate, rat spleen tissue lysate, RAW264.7 cell lysate, human colon tissue, mouse spleen tissue, HeLa.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:500
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 25 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SOCS3
SYNONYMS
ATOD4 antibody;CIS 3 antibody;CIS-3 antibody;CIS3 antibody;Cish3 antibody;Cytokine induced SH2 protein 3 antibody;Cytokine-inducible SH2 protein 3 antibody;E2a Pbx1 target gene in fibroblasts 10 antibody;EF 10 antibody;MGC71791 antibody;SOCS 3 antibody;SOCS-3 antibody;Socs3 antibody;SOCS3_HUMAN antibody;SSI 3 antibody;SSI-3 antibody;SSI3 antibody;STAT induced STAT inhibitor 3 antibody;STAT-induced STAT inhibitor 3 antibody;Suppressor of cytokine signaling 3 antibody
SUBCELLULAR LOCATION
Cytoplasmic side of plasma membrane
FUNCTION
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
CITATIONS
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Mettl14-mediated m6A modification enhances the function of Foxp3+ regulatory T cells and promotes allograft acceptance
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WB
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Mouse
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Citation
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