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ERK2 Recombinant Mouse Monoclonal Antibody [C3B12-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610079

ERK2 Recombinant Mouse Monoclonal Antibody [C3B12-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ERK2 Recombinant Mouse Monoclonal Antibody [C3B12-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human ERK2 aa 200-360.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 41 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

C3B12-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

Background References

1. Wang VY. et. al. Bcl3 Phosphorylation by Akt, Erk2, and IKK Is Required for Its Transcriptional Activity. Mol Cell. 2017 Aug 3;67(3):484-497.e5.

2. Schwebs DJ. et. al. Dictyostelium Erk2 is an atypical MAPK required for chemotaxis. Cell Signal. 2018 Jun;46:154-165.

Subcellular Location

Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.

UNIPROT

SwissProt: P28482 Human

SwissProt: P63085 Mouse

SwissProt: P63086 Rat

Synonyms

ERK 2 antibody

ERK-2 antibody

ERT1 antibody

Extracellular Signal Regulated Kinase 2 antibody

Extracellular signal-regulated kinase 2 antibody

MAP kinase 1 antibody

MAP kinase 2 antibody

MAP kinase isoform p42 antibody

MAPK 1 antibody

MAPK 2 antibody

Expand

Images

  • This data was developed using <a href="/products/HA601201" style="font-weight: bold;text-decoration: underline;">HA601201</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (<a href="/products/HA601201" style="font-weight: bold;text-decoration: underline;">HA601201</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: A431 cell lysate (20 µg/Lane)<br />Lane 5: HepG2 cell lysate (20 µg/Lane)<br />Lane 6: HEK-293 cell lysate (20 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 8: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 9: C6 cell lysate (20 µg/Lane)<br />Lane 10: Human brain tissue lysate (40 µg/Lane)<br />Lane 11: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 12: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 43 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601201" style="font-weight: bold;text-decoration: underline;">HA601201</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/001-100-005" style="font-weight: bold;text-decoration: underline;">001-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA601201, the same antibody clone in a different buffer formulation.
    Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA601201) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: A431 cell lysate (20 µg/Lane)
    Lane 5: HepG2 cell lysate (20 µg/Lane)
    Lane 6: HEK-293 cell lysate (20 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 8: RAW264.7 cell lysate (20 µg/Lane)
    Lane 9: C6 cell lysate (20 µg/Lane)
    Lane 10: Human brain tissue lysate (40 µg/Lane)
    Lane 11: Mouse brain tissue lysate (40 µg/Lane)
    Lane 12: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 43 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601201) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />This data was developed using <a href="/products/HA601201" style="font-weight: bold;text-decoration: underline;">HA601201</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (<a href="/products/HA601201" style="font-weight: bold;text-decoration: underline;">HA601201</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate (10 µg/Lane)<br />Lane 2: A549-si ERK2 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601201" style="font-weight: bold;text-decoration: underline;">HA601201</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    This data was developed using HA601201, the same antibody clone in a different buffer formulation.
    Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA601201) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate (10 µg/Lane)
    Lane 2: A549-si ERK2 cell lysate (10 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601201) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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