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ERK2 Recombinant Mouse Monoclonal Antibody [5-D2-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610080

ERK2 Recombinant Mouse Monoclonal Antibody [5-D2-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ERK2 Recombinant Mouse Monoclonal Antibody [5-D2-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human ERK2 aa aa 200-359.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 41 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

5-D2-R

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis.

Background References

1. Wortzel I et al. The ERK cascade: distinct functions within various subcellular organelles. Genes Cancer 2:195-209 (2011).

2. Ohori M et al. Role of a cysteine residue in the active site of ERK and the MAPKK family. Biochem Biophys Res Commun 353:633-637 (2007).

3. Ohori M et al. Identification of a selective ERK inhibitor and structural determination of the inhibitor-ERK2 complex. Biochem Biophys Res Commun 336:357-363 (2005).

Subcellular Location

Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.

UNIPROT

SwissProt: P28482 Human

SwissProt: P63085 Mouse

SwissProt: P63086 Rat

Synonyms

ERK 2 antibody

ERK-2 antibody

ERT1 antibody

Extracellular Signal Regulated Kinase 2 antibody

Extracellular signal-regulated kinase 2 antibody

MAP kinase 1 antibody

MAP kinase 2 antibody

MAP kinase isoform p42 antibody

MAPK 1 antibody

MAPK 2 antibody

Expand

Images

  • Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (<a href="/products/HA610080" style="font-weight: bold;text-decoration: underline;">HA610080</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: A431 cell lysate (20 µg/Lane)<br />Lane 5: HepG2 cell lysate (20 µg/Lane)<br />Lane 6: HEK-293 cell lysate (20 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 8: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 9: C6 cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610080" style="font-weight: bold;text-decoration: underline;">HA610080</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/001-100-005" style="font-weight: bold;text-decoration: underline;">001-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA610080) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: A431 cell lysate (20 µg/Lane)
    Lane 5: HepG2 cell lysate (20 µg/Lane)
    Lane 6: HEK-293 cell lysate (20 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 8: RAW264.7 cell lysate (20 µg/Lane)
    Lane 9: C6 cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610080) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (<a href="/products/HA610080" style="font-weight: bold;text-decoration: underline;">HA610080</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (40 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 3: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 1 minute 2 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610080" style="font-weight: bold;text-decoration: underline;">HA610080</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/001-100-005" style="font-weight: bold;text-decoration: underline;">001-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA610080) at 1/1,000 dilution.

    Lane 1: Human brain tissue lysate (40 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)
    Lane 3: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 1 minute 2 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610080) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (<a href="/products/HA610080" style="font-weight: bold;text-decoration: underline;">HA610080</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate (10 µg/Lane)<br />Lane 2: A549-si ERK2 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610080" style="font-weight: bold;text-decoration: underline;">HA610080</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA610080) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate (10 µg/Lane)
    Lane 2: A549-si ERK2 cell lysate (10 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610080) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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