ESD Rabbit Polyclonal Antibody
Catalog# R1511-9
ESD Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
ESD Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human ESD aa 1-282 / 282.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 31 kDa
Positive Control
K562 cell lysate, mouse intestine tissue lysate, Hela, LOVO, SW480, human prostate tissue, human pancreas tissue, mouse colon tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
ESD (esterase D) is also known as S-formylglutathione hydrolase and is a 282 amino acid protein that is a member of the esterase D family. ESD is highly expressed in placenta, kidney, liver and erythrocytes, and is localized to the cytoplasm, as well as to cytoplasmic vesicles. The main function of ESD is to detoxify formaldehyde while providing energy. Formaldehyde is oxidized by ADH5 which yields S-formylglutathione. ESD then catalyzes the hydrolysis of S-formylglutathione to the reduced forms of formic acid and glutathione. In addition, ESD hydrolyzes a variety of different neutral ester substrates and can act as a carboxylesterase. ESD may also act as a cysteine hydrolase which is inactivated by thiol alkylating agents. ESD gene polymorphism can lead to reduced enzymatic activity which may cause susceptibility to many conditions, including toxic liver cirrhosis, retinoblastoma, obesity and autism.
Background References
暂无
Sequence Similarity
Belongs to the esterase D family.
Subcellular Location
Cytoplasm, Cytoplasmic vesicle.
Synonyms
EC 3.1.2.12 antibody
Es-10 antibody
Es10 antibody
ESD antibody
ESTD_HUMAN antibody
Esterase 10 antibody
Esterase D antibody
Esterase D formylglutathione hydrolase antibody
FGH antibody
FGH, included antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of ESD on different lysates with Rabbit anti-ESD antibody (R1511-9) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate (10 µg/Lane)
Lane 2: HAP1-ESD KD cell lysate (10 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-9) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ESD on K562 cell (1) and mouse intestine tissue (2) lysate using anti-ESD antibody at 1/1,000 dilution.
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ICC staining ESD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining ESD in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining ESD in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ESD antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ESD antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-e=ESD antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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