Skip to content

LRP1 Recombinant Rabbit Monoclonal Antibody [SA0290]

Usd: 205

Specification

Bulk Order Quote | Customization Request

Catalog# ET1601-1

LRP1 Recombinant Rabbit Monoclonal Antibody [SA0290]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LRP1 Recombinant Rabbit Monoclonal Antibody [SA0290]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human LRP1 aa 4,471-4,520 / 4,544.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P, IP, IHC-Fr

Molecular Weight

Predicted band size: 505 kDa

Positive Control

Mouse liver tissue lysate, Human lung tissue lysate, Rat liver tissue lysate, Rat lung tissue lysate, Mouse lung tissue lysate, Human liver tissue lysate, mouse brain tissue, rat brain tissue, rat liver tissue, human lung tissue, human liver tissue, mouse liver tissue.

Conjugation

unconjugated

Clone Number

SA0290

RRID

AB_3069592

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:50

  • IHC-P

  • 1:200-1:2,000

  • IP

  • 1-2μg/sample

  • IHC-Fr

  • 1:100

Target

Function

LRP1 is a member of the LDLR family and ubiquitously expressed in multiple tissues, though it is most abundant in vascular smooth muscle cells (SMCs), hepatocytes, and neurons. LRP1 plays a key role in intracellular signaling and endocytosis, which thus implicate it in many cellular and biological processes, including lipid and lipoprotein metabolism, protease degradation, platelet derived growth factor receptor regulation, integrin maturation and recycling, regulation of vascular tone, regulation of blood brain barrier permeability, cell growth, cell migration, inflammation, and apoptosis, as well as diseases such as neurodegenerative diseases, atherosclerosis, and cancer. The LRP1 gene encodes a 600 kDa precursor protein that is processed by furin in the trans-Golgi complex, resulting in a 515 kDa alpha-chain and an 85 kDa beta-chain associated noncovalently.

Background References

1. Fernandez-Castaneda, A. et al. 2013. Identification of the low density lipoprotein (LDL) receptor-related protein-1 interactome in central nervous system myelin suggests a role in the clearance of necrotic cell debris. J. Biol. Chem. 288: 4538-4548.

2. Yahiro, K. et al. 2012. Low-density lipoprotein receptor-related protein-1 (LRP1) mediates autophagy and apoptosis caused by Helicobacter pylori VacA. J. Biol. Chem. 287: 31104-31115.

Sequence Similarity

Belongs to the LDLR family.

Tissue Specificity

Most abundant in liver, brain and lung.

Post-translational Modification

Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.; The N-terminus is blocked.; Phosphorylated on serine and threonine residues.; Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.

Subcellular Location

Cytoplasm, Nucleus, Membrane.

UNIPROT

SwissProt: Q07954 Human

SwissProt: Q91ZX7 Mouse

SwissProt: G3V928 Rat

Synonyms

A2MR antibody

Alpha 2 macroglobulin receptor antibody

alpha 2MR antibody

Alpha-2-macroglobulin receptor antibody

APOER antibody

Apolipoprotein E receptor antibody

APR antibody

CD 91 antibody

CD91 antibody

CD91 antigen antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />All lanes: Western blot analysis of LRP1 with anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1:1,000 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2/3: LRP1 knockdown Hela whole cell lysate (10 µg).<br /><br /><a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a> was shown to specifically react with LRP1 in wild-type Hela cells. Weakened bands were observed when LRP1 knockdown samples were tested. Wild-type and LRP1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    All lanes: Western blot analysis of LRP1 with anti-LRP1 antibody (ET1601-1) at 1:1,000 dilution.
    Lane 1: Wild-type Hela whole cell lysate (10 µg).
    Lane 2/3: LRP1 knockdown Hela whole cell lysate (10 µg).

    ET1601-1 was shown to specifically react with LRP1 in wild-type Hela cells. Weakened bands were observed when LRP1 knockdown samples were tested. Wild-type and LRP1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1601-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of frozen mouse hippocampus tissue labeling LRP1 with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>).<br />The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

    Immunofluorescence analysis of frozen mouse hippocampus tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1).
    The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

  • Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling LRP1 with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>).<br />The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

    Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1).
    The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

  • LRP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    LRP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1601-1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-1 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: ET1601-1 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of ET1601-1 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes; ECL: K1801

  • Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (<a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Rat liver tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br />Exposure time: 5 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1601-1" style="font-weight: bold;text-decoration: underline;">ET1601-1</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/5,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 504.6 kDa<br />Observed band size: 80 kDa

    Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate

    Lysates/proteins at 40 µg/Lane.
    Exposure time: 5 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1601-1, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/5,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 504.6 kDa
    Observed band size: 80 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation