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Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]

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Specification

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Catalog# ET1601-21

Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Alkaline Phosphatase aa 18-50.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Saos-2 cell lysate, HeLa cell lysate, A549 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HepG2, SW480, mouse kidney tissue, mouse jawbone tissue, rat liver tissue, mouse liver tissue, Hela.

Conjugation

unconjugated

Clone Number

SA40-00

RRID

AB_3069604

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000-1:8,000

  • IF-Tissue

  • 1:200

Target

Function

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.

Background References

1. Chen M. et. al. Low-dose X-ray irradiation promotes osteoblast proliferation, differentiation and fracture healing. PLoS One 9:e104016 (2014).

Sequence Similarity

Belongs to the alkaline phosphatase family.

Post-translational Modification

N-glycosylated.

Subcellular Location

Cell membrane, Mitochondrion membrane, Mitochondrion intermembrane space, Extracellular vesicle membrane.

UNIPROT

SwissProt: P05186 Human

SwissProt: P09242 Mouse

SwissProt: P08289 Rat

Synonyms

Alkaline phosphatase antibody

Alkaline phosphatase placental antibody

Alkaline phosphatase placental type antibody

Alkaline phosphatase Regan isozyme antibody

ALP antibody

Alp1 antibody

ALPP antibody

FLJ61142 antibody

Germ-cell alkaline phosphatase antibody

nagao isozyme antibody

Expand

Images

  • Western blot analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/5,000 dilution.<br /><br />Lane 1: Saos-2 cell lysate (15 µg/Lane)<br />Lane 2: HeLa cell lysate (15 µg/Lane)<br />Lane 3: A549 cell lysate (15 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 57 kDa<br />Observed band size: 75 kDa<br />Exposure time: 24 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/5,000 dilution.

    Lane 1: Saos-2 cell lysate (15 µg/Lane)
    Lane 2: HeLa cell lysate (15 µg/Lane)
    Lane 3: A549 cell lysate (15 µg/Lane)
    Lane 4: Mouse liver tissue lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (20 µg/Lane)

    Predicted band size: 57 kDa
    Observed band size: 75 kDa
    Exposure time: 24 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse jawbone tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse jawbone tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: liver <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: liver

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: bone<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Mouse

    Site: bone

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: bone<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Mouse

    Site: bone

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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