Skip to content

Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1601-26

Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Alas1 aa 510-640 / 640.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 71 kDa

Positive Control

A549 cell lysate, HepG2 cell lysate, THP-1 cell lysate, HeLa cell lysate, JAR cell lysate, Raji cell lysate, Hela, HepG2, MCF-7.

Conjugation

unconjugated

Clone Number

SA70-04

RRID

AB_3069608

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Delta-aminolevulinate synthase 1 also known as ALAS1 is a protein that in humans is encoded by the ALAS1 gene. ALAS1 is an aminolevulinic acid synthase. Delta-aminolevulinate synthase catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2. Mice lacking this gene exhibit embryonic embryonic lethality, indicating that ALAS is essential for early embryogenesis.

Background References

1. The MGC Project Team. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14:2121-2127(2004).

2. Bishop D.F. Two different genes encode delta-aminolevulinate synthase in humans: nucleotide sequences of cDNAs for the housekeeping and erythroid genes. Nucleic Acids Res. 18:7187-7188(1990).

Sequence Similarity

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Subcellular Location

Mitochondrion matrix

UNIPROT

SwissProt: P13196 Human

Synonyms

5 aminolevulinate synthase antibody

5 aminolevulinate synthase nonspecific mitochondrial antibody

5 aminolevulinic acid synthase antibody

5-aminolevulinate synthase antibody

5-aminolevulinic acid synthase 1 antibody

Alas 1 antibody

ALAS 3 antibody

ALAS antibody

ALAS H antibody

ALAS HOUSEKEEPING TYPE antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD Alas1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 71 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD Alas1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 71 kDa
    Observed band size: 65 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: THP-1 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: JAR cell lysate<br />Lane 5: Raji cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 71 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: JAR cell lysate
    Lane 5: Raji cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 71 kDa
    Observed band size: 65 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling Alas1 with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of HepG2 cells labeling Alas1 with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HepG2 cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of MCF-7 cells labeling Alas1 with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (<a href="/products/ET1601-26" style="font-weight: bold;text-decoration: underline;">ET1601-26</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of MCF-7 cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"