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JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06]

Usd: 385

Specification

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Catalog# ET1601-28

JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cow

  • Monkey

  • Zebrafish

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human JNK1 aa 1-423 / 427.

Species Reactivity

Human, Mouse, Rat (Predicted: Cow, Monkey, Zebrafish)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 48/53 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

SA43-06

RRID

AB_3069610

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:200

  • IHC-P

  • 1:500-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

The c-Jun N-terminal kinases consist of ten isoforms derived from three genes: JNK1 (four isoforms), JNK2 (four isoforms) and JNK3 (two isoforms). Each gene is expressed as either 46 kDa or 55 kDa protein kinases, depending upon how the 3' coding region of the corresponding mRNA is processed. There have been no functional differences documented between the 46 kDa and the 55 kDa isoform, however, a second form of alternative splicing occurs within transcripts of JNK1 and JNK2, yielding JNK1-α, JNK2-α and JNK1-β and JNK2-β. Differences in interactions with protein substrates arise because of the mutually exclusive utilization of two exons within the kinase domain. and JNK2 are found in all cells and tissues. JNK3 is found mainly in the brain, but is also found in the heart and the testes.

Background References

1. Cantrell, M. et al. 2015. c-Jun N-terminal kinase 2 prevents luminal cell commitment in normal mammary glands and tumors by inhibiting p53/Notch1 and breast cancer gene 1 expression. Oncotarget. 6: 11863-11881.

2. Marampon, F. et al. 2015. Vitamin D protects endothelial cells from irradiation-induced senescence and apoptosis by modulating MAPK/SirT1 axis. Journal of endocrinological investigation.

Sequence Similarity

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Post-translational Modification

Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1. Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity).

Subcellular Location

Cytoplasm, Nucleus, Membrane, Mitochondrion

UNIPROT

SwissProt: P45983 Human

SwissProt: P45984 Human

SwissProt: P53779 Human

SwissProt: Q61831 Mouse

SwissProt: Q91Y86 Mouse

SwissProt: Q9WTU6 Mouse

SwissProt: P49185 Rat

SwissProt: P49186 Rat

SwissProt: P49187 Rat

Synonyms

C Jun kinase 2 antibody

c Jun N terminal kinase 1 antibody

c Jun N terminal kinase 2 antibody

c Jun N terminal kinase 3 antibody

c-Jun N-terminal kinase 1 antibody

JNK 46 antibody

JNK 55 antibody

JNK antibody

JNK-46 antibody

JNK1 antibody

Expand

Images

  • Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: PC-12 cell lysate<br />Lane 7: C6 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 48/53 kDa<br />Observed band size: 48/53 kDa<br /><br />Exposure time: 35 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: C6 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 48/53 kDa
    Observed band size: 48/53 kDa

    Exposure time: 35 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (15 µg/Lane)<br />Lane 2: Rat brain tissue lysate (15 µg/Lane)<br /><br />Predicted band size: 48/53 kDa<br />Observed band size: 48/53 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (15 µg/Lane)
    Lane 2: Rat brain tissue lysate (15 µg/Lane)

    Predicted band size: 48/53 kDa
    Observed band size: 48/53 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • JNK1+JNK2+JNK3 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with <a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HEK-293 cell lysate (input)<br />Lane 2: <a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a> IP in HEK-293 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1601-28" style="font-weight: bold;text-decoration: underline;">ET1601-28</a> in HEK-293 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (K1803)<br />Exposure time: 7 seconds; ECL: K1801

    JNK1+JNK2+JNK3 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with ET1601-28 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-28 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HEK-293 cell lysate (input)
    Lane 2: ET1601-28 IP in HEK-293 cell lysate
    Lane 3: Rabbit IgG instead of ET1601-28 in HEK-293 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 7 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP

Reactivity: Human,Mouse,Rat,Cow,Monkey,Zebrafish

Conjugate: unconjugated