Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]
Catalog# ET1602-46
Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]
-
WB
-
IP
-
Species independent
-
unconjugated
Overview
Product Name
Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Escherichia coli MBP aa 331-380 / 396.
Species Reactivity
Species independent
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
Recombinant MBP-tag protein.
Conjugation
unconjugated
Clone Number
SR41-04
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IP
-
2-5 µg/ml.
Target
Function
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.
Background References
1. Ishida K et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014).
2. Beck J et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013).
Sequence Similarity
Belongs to the bacterial solute-binding protein 1 family.
Synonyms
ECK4026 antibody
JW3994 antibody
Mal E antibody
MalE antibody
malJ antibody
Maltodextrin binding protein antibody
Maltose ABC transporter periplasmic protein antibody
Maltose binding periplasmic protein antibody
MMBP antibody
Periplasmic maltose binding protein antibody
Images
-
MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with ET1602-46 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1602-46 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 60 mins at room temperature.
Lane 1: MBP Tag fusion protein lysate (input).
Lane 2: ET1602-46 IP in MBP Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST -
Western blot analysis of Maltose Binding Protein on MBP-tag only cell lysate with Rabbit anti-Maltose Binding Protein antibody (ET1602-46) at 1/1,000 dilution.
Lysates/proteins at 5 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1602-46, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 43 kDa
Observed band size: 43 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"