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Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]

Usd: 385

Specification

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Catalog# ET1604-45

Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]

Application

  • WB

  • IHC-P

  • IP

  • FC

  • IF-Tissue

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HO-1 aa 227-276 / 288.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP, FC, IF-Tissue

Molecular Weight

Predicted band size: 33 kDa

Positive Control

Human spleen tissue, human liver tissue, Jurkat.

Conjugation

unconjugated

Clone Number

SP08-07

RRID

AB_3069700

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:500-1:1,000

  • IP

  • Use at an assay dependent concentration.

  • IF-Tissue

  • 1:500

Target

Function

Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.

Background References

1. He C et al. Vasoprotective effect of PDGF-CC mediated by HMOX1 rescues retinal degeneration. Proc Natl Acad Sci U S A 111:14806-11 (2014).

2. Maruyama A et al. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res 42:13599-614 (2014).

Sequence Similarity

Belongs to the heme oxygenase family.

Tissue Specificity

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: P09601 Human

Synonyms

32 kD antibody

bK286B10 antibody

D8Wsu38e antibody

heat shock protein 32 kD antibody

heat shock protein 32kD antibody

Heat shock protein antibody

Heme oxygenase (decycling) 1 antibody

Heme oxygenase 1 antibody

Hemox antibody

HMOX 1 antibody

Expand

Images

  • Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1 (HO-1).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1 (HO-1).

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1604-45, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (ET1604-45) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (ET1604-45) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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