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TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06]

Usd: 385

Specification

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Catalog# ET1605-18

TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TACC3 aa 40-89 / 838.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 90 kDa

Positive Control

HT29 cell lysates, Hela cell lysates,Hela, HepG2, human tonsil tissue, human spleen tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

SY10-06

RRID

AB_3069706

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

TACC1 (transforming acidic coiled coil gene 1) is one of three TACC family members, which are thought to be involved in breast tumorigenesis. TACC1 is located on 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1 whose expression is enhanced in most breast cancer tumors. TACC family members, TACC1, TACC2, and TACC3, map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog, D-TACC. It has been shown that D-TACC is necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner.

Background References

1. Zhou H., et al. 2013. Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. 12:260-271.

2. Rigbolt K.T., et al. 2011. System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci. Signal. 4:RS3-RS3.

Sequence Similarity

Belongs to the TACC family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9Y6A5 Human

SwissProt: Q9JJ11 Mouse

Unigene: 58838 Rat

Synonyms

ERIC 1 antibody

ERIC-1 antibody

ERIC1 antibody

MGC117382 antibody

MGC133242 antibody

OTTHUMP00000113796 antibody

TACC3 antibody

TACC3_HUMAN antibody

Transforming acidic coiled coil containing protein 3 antibody

Transforming acidic coiled-coil-containing protein 3 antibody

Images

  • Western blot analysis of TACC3 on different lysates with Rabbit anti-TACC3 antibody (<a href="/products/ET1605-18" style="font-weight: bold;text-decoration: underline;">ET1605-18</a>) at 1/500 dilution.<br /><br />Lane 1: HT29 cell lysates<br />Lane 2: Hela cell lysates<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 90 kDa<br />Observed band size: 140 kDa<br /><br />Exposure time: 2 minutes;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-18" style="font-weight: bold;text-decoration: underline;">ET1605-18</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TACC3 on different lysates with Rabbit anti-TACC3 antibody (ET1605-18) at 1/500 dilution.

    Lane 1: HT29 cell lysates
    Lane 2: Hela cell lysates

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 90 kDa
    Observed band size: 140 kDa

    Exposure time: 2 minutes;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining TACC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TACC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TACC3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TACC3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"