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CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]

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Specification

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Catalog# ET1605-4

CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

  • CUT&Tag-seq

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CDX2 aa 20-60.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, CUT&Tag-seq

Molecular Weight

Predicted band size: 34 kDa

Positive Control

Caco-2 cell lysate, LoVo cell lysate, AGS cell lysate, LOVO, AGS, human appendix tissue, Human stomach cancer tissue, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue, Hela.

Conjugation

unconjugated

Clone Number

SY09-02

RRID

AB_3069712

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:500-1:2,000

  • IHC-P

  • 1:5,000-1:20,000

  • FC

  • 1:1,000

Target

Function

In common with the two other Cdx genes, CDX2 regulates several essential processes in the development and function of the lower gastrointestinal tract (from the duodenum to the anus) in vertebrates. In vertebrate embryonic development, CDX2 becomes active in endodermal cells that are posterior to the developing stomach.These cells eventually form the intestinal epithelium. The activity of CDX2 at this stage is essential for the correct formation of the intestine and the anus.CDX2 is also required for the development of the placenta.

Background References

1. Yang Y et al. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure. Proc Natl Acad Sci U S A 112:E2337-46 (2015).

2. Ohinata Y & Tsukiyama T Establishment of trophoblast stem cells under defined culture conditions in mice. PLoS One 9:e107308 (2014).

Sequence Similarity

Belongs to the Caudal homeobox family.

Post-translational Modification

Phosphorylation of Ser-60 mediates the transactivation capacity.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q99626 Human

SwissProt: P43241 Mouse

Entrez Gene: 66019 Rat

Synonyms

Caudal type homeo box 2 antibody

Caudal type homeo box transcription factor 2 antibody

Caudal type homeobox 2 antibody

Caudal type homeobox protein 2 antibody

Caudal type homeobox transcription factor 2 antibody

Caudal-type homeobox protein 2 antibody

CDX 2 antibody

CDX 3 antibody

CDX-3 antibody

Cdx2 antibody

Expand

Images

  • Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: LoVo cell lysate<br />Lane 3: AGS cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (ET1605-4) at 1/1,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: LoVo cell lysate
    Lane 3: AGS cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7-si NT cell lysate<br />Lane 2: MCF7-si CDX2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (ET1605-4) at 1/1,000 dilution.

    Lane 1: MCF7-si NT cell lysate
    Lane 2: MCF7-si CDX2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Human<br />Sample: Caco-2 (Human colorectal adenocarcinoma cell)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br /><br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br />Primary antibody: <a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>, 1/2,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature.<br /><br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: Caco-2 (Human colorectal adenocarcinoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: ET1605-4, 1/2,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

    Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

  • Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution.<br /><br />Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with <a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a> for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND&reg; RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ET1605-4 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human thymus tissue (negative) with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue (negative) with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat thymus tissue (negative) with Rabbit anti-CDX2 antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue (negative) with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Human<br />Sample: Caco-2 (Human Caucasian colon adenocarcinoma)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.<br /><br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br />Primary antibody: <a href="/products/ET1605-4" style="font-weight: bold;text-decoration: underline;">ET1605-4</a>(1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: Caco-2 (Human Caucasian colon adenocarcinoma)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.
    Primary antibody: ET1605-4(1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation