Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]
Catalog# ET1607-16
Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]
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WB
-
IP
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Caveolin-3 aa 1-125 / 151.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP, IHC-P
Molecular Weight
17 kDa
Positive Control
Mouse heart tissue lysate, mouse skeletal muscle tissue lysate, rat heart tissue, human striated muscle tissue.
Conjugation
unconjugated
Clone Number
SY22-06
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
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IHC-P
-
1:1,000
Target
Function
Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.
Background References
1. Niesman IR et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014).
2. Zheng J et al. Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog. PLoS One 9:e94732 (2014).
Sequence Similarity
Belongs to the caveolin family.
Tissue Specificity
Expressed predominantly in muscle.
Post-translational Modification
Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2.
Subcellular Location
Golgi apparatus membrane, Cell membrane, Membrane.
Synonyms
CAV3 antibody
CAV3_HUMAN antibody
Caveolin 3 antibody
Caveolin-3 antibody
LGMD1C antibody
LQT9 antibody
M-caveolin antibody
MGC126100 antibody
MGC126101 antibody
MGC126129 antibody
ExpandImages
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Western blot analysis of Caveolin-3 on different lysates using anti-Caveolin-3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse heart tissue lysates
Lane 2: Mouse skeletal muscle tissue lysates -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"