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JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]

Usd: 385

Specification

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Catalog# ET1607-35

JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal human JAK2.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 131 kDa

Positive Control

TF-1 cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Jurkat cell lysate, MEF cell lysate, A549, MCF-7, NIH/3T3.

Conjugation

unconjugated

Clone Number

SY0245

RRID

AB_3069760

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100-1:500

Target

Function

JAK2 (Janus kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

Background References

1. Gan, X.T. et al. 2015. Myocardial Hypertrophic Remodeling and Impaired Left Ventricular Function in Mice with a Cardiac-Specific Deletion of Janus Kinase 2. The American journal of pathology. 185: 3202-10.

2. Tang, H. et al. 2011. Changes in growth hormone (GH), GH receptor, and GH signal transduction in hippocampus of congenital hypothyroid rats. J. Neurosci. Res. 89: 248-255.

Sequence Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Tissue Specificity

Ubiquitously expressed throughout most tissues.

Post-translational Modification

Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.

Subcellular Location

Endomembrane system, Cytoplasm, Nucleus.

UNIPROT

SwissProt: O60674 Human

SwissProt: Q62120 Mouse

SwissProt: Q62689 Rat

Synonyms

JAK 2 antibody

JAK-2 antibody

JAK2 antibody

JAK2_HUMAN antibody

Janus Activating Kinase 2 antibody

Janus kinase 2 (a protein tyrosine kinase) antibody

Janus kinase 2 antibody

JTK 10 antibody

JTK10 antibody

kinase Jak2 antibody

Expand

Images

  • Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution.<br /><br />Lane 1: TF-1 cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: THP-1 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: C6 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 5 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution.

    Lane 1: TF-1 cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: C6 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 120 kDa

    Exposure time: 5 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si JAK2 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 45 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si JAK2 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 120 kDa

    Exposure time: 45 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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