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VPS34 Recombinant Rabbit Monoclonal Antibody [SY0286]

Usd: 385

Specification

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Catalog# ET1607-74

VPS34 Recombinant Rabbit Monoclonal Antibody [SY0286]

Application

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

VPS34 Recombinant Rabbit Monoclonal Antibody [SY0286]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human VPS34 aa 830-870.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, FC

Molecular Weight

Predicted band size: 102 kDa

Positive Control

Human stomach cancer tissue, mouse brain tissue, rat brain tissue, A549.

Conjugation

unconjugated

Clone Number

SY0286

RRID

AB_3069781

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:1,000

Target

Function

Phosphatidylinositol 3-kinase catalytic subunit type 3 is an enzyme subunit that in humans is encoded by the PIK3C3 gene. It's a class III phosphoinositide 3-kinase. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. PI 3-kinase p100 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. PI 3-kinase p100 exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. Unlike class I PI3Ks, whose activities are enhanced in the presence of magnesium, PI 3-kinase p100 activity is enhanced by manganese. Its activity can also be regulated by nutrients, suggesting an important role of PI-3 kinase p100 in the regulation of mTOR protein synthesis and autophagy.

Background References

1. Bechtel W et al.2013. Vps34 deficiency reveals the importance of endocytosis for podocyte homeostasis. J Am Soc Nephrol 24:727-43.

2. Liu, MN. et al. 2015. Functional mechanism of the enhancement of 5-fluorouracil sensitivity by TUSC4 in colon cancer cells. Oncol Lett. 10: 3682-3688.

Sequence Similarity

Belongs to the PI3/PI4-kinase family.

Tissue Specificity

Ubiquitously expressed, with a highest expression in skeletal muscle.

Subcellular Location

Midbody, Late endosome, Cytoplasmic vesicle.

UNIPROT

SwissProt: Q8NEB9 Human

SwissProt: Q6PF93 Mouse

SwissProt: O88763 Rat

Synonyms

hVps34 antibody

MGC61518 antibody

Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody

Phosphatidylinositol 3 kinase class 3 antibody

Phosphatidylinositol 3 kinase p100 subunit antibody

Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody

Phosphatidylinositol 3-kinase p100 subunit antibody

Phosphoinositide 3 kinase class 3 antibody

Phosphoinositide-3-kinase class 3 antibody

PI3 kinase type 3 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-VPS34 antibody (<a href="/products/ET1607-74" style="font-weight: bold;text-decoration: underline;">ET1607-74</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1607-74" style="font-weight: bold;text-decoration: underline;">ET1607-74</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-VPS34 antibody (ET1607-74) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VPS34 antibody (<a href="/products/ET1607-74" style="font-weight: bold;text-decoration: underline;">ET1607-74</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1607-74" style="font-weight: bold;text-decoration: underline;">ET1607-74</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VPS34 antibody (ET1607-74) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VPS34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1607-74" style="font-weight: bold;text-decoration: underline;">ET1607-74</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VPS34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of A549 cells labeling VPS34.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1607-74" style="font-weight: bold;text-decoration: underline;">ET1607-74</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A549 cells labeling VPS34.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1607-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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metafield image

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