PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68] (ET1608-56)
Catalog# ET1608-56
Rabbit Monoclonal to PARP1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: C2C12 cell lysate (15 µg/Lane)
Lane 5: C6 cell lysate (15 µg/Lane)
Lane 6: PC-12 cell lysate (15 µg/Lane)
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68] (ET1608-56)
Immunogen
Synthetic peptide within n-terminal human parp1.
Host
Rabbit
Positive Control
A549 cell lysate, Jurkat cell lysate, Hela cell lysate, HeLa, human spleen tissue, mouse large intestine tissue, human breast carcinoma tissue, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU03-68
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:500
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IF-Tissue
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1:50-1:800
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IHC-P
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1:500-1:4,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 113 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PARP1
SYNONYMS
ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody;ADP ribosyltransferase diphtheria toxin like 1 antibody;ADP ribosyltransferase NAD(+) antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;ARTD1 antibody;msPARP antibody;NAD(+) ADP ribosyltransferase 1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;pADPRT 1 antibody;pADPRT1 antibody;PARP 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly (ADP ribose) polymerase 1 antibody;poly (ADP ribose) polymerase family, member 1 antibody;Poly [ADP-ribose] polymerase 1 antibody;Poly(ADP ribose) polymerase antibody;poly(ADP ribose) synthetase antibody;poly(ADP ribosyl)transferase antibody;Poly[ADP ribose] synthetase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.
SUBCELLULAR LOCATION
Nucleus, nucleolus, chromosome.
FUNCTION
The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.
CITATIONS
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Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway. Biochemical pharmacology, 186, 114475.
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Dr Xia Liu,Professor Jianchao Wang
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Fang Xiao,et al
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Human
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Human
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Citation
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WB
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IHC
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human
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Citation
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Wang, F., Liu, Y., Ni, F., Jin, J., Wu, Y., Huang, Y., Ye, X., Shen, X., Ying, Y., Chen, J., Chen, R., Zhang, Y., Sun, X., Wang, S., Xu, X., Chen, C., Guo, J., & Zhang, D.
BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency
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Mouse
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Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L.
Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells
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Human
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Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G.
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma
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Human
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Chaetocin exhibits anticancer effects in esophageal squamous cell carcinoma via activation of Hippo pathway
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