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Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07]

Usd: 205

Specification

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Catalog# ET1608-69

Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07]

Application

  • WB

  • FC

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Caspase-1 aa 213-404 / 404.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC, IF-Cell

Molecular Weight

Predicted band size: 45 kDa

Positive Control

THP-1 cell lysate, HCT 116 cell lysate, HL-60 cell lysate, RAW264.7 cell lysate, Mouse spleen lysate, Rat spleen lysate, U937 cell lysates, PC-12, HL-60, THP-1.

Conjugation

unconjugated

Clone Number

SU40-07

RRID

AB_2940740

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:50,000-1:100,000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.

Background References

1. Zhao J et al. Synthetic Oligodeoxynucleotides Containing Multiple Telemeric TTAGGG Motifs Suppress Inflammasome Activity in Macrophages Subjected to Oxygen and Glucose Deprivation and Reduce Ischemic Brain Injury in Stroke-Prone Spontaneously Hypertensive Rats. PLoS One 10:e0140772 (2015).

2. Zhang X et al. Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo. BMC Vet Res 11:264 (2015).

Sequence Similarity

Belongs to the peptidase C14A family.

Tissue Specificity

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

Post-translational Modification

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: P29466 Human

SwissProt: P29452 Mouse

SwissProt: P43527 Rat

Synonyms

Caspase1

CASP-1 antibody

CASP1 antibody

CASP1_HUMAN antibody

Caspase1 antibody

Caspase 1 antibody

Caspase-1 subunit p10 antibody

ICE antibody

IL-1 beta-converting enzyme antibody

IL-1BC antibody

Expand

Images

  • Western blot analysis of Caspase-1 on different lysates with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: HCT 116 cell lysate<br />Lane 3: HL-60 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: Mouse spleen lysate<br />Lane 6: Rat spleen lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caspase-1 on different lysates with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: HCT 116 cell lysate
    Lane 3: HL-60 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: Mouse spleen lysate
    Lane 6: Rat spleen lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-69) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Caspase-1 on U937 cell lysates with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/50,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caspase-1 on U937 cell lysates with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/50,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-69) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HL-60 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100 dilution.<br /><br />Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HL-60 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution.

    Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of PC-12 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of THP-1 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100 dilution.<br /><br />Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of THP-1 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution.

    Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of PC-12 cells labeling Caspase-1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1608-69" style="font-weight: bold;text-decoration: underline;">ET1608-69</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PC-12 cells labeling Caspase-1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-69, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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