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beta II Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04]

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Specification

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Catalog# ET1609-48

beta II Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Human

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

beta II Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human beta Ⅱ Tubulin aa 407-445 / 445.

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 50 kDa

Positive Control

C2C12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse kidney tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

ST52-04

RRID

AB_3069858

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.

Background References

1. Gill, BC. et al. 2013. Neurotrophin therapy improves recovery of the neuromuscular continence mechanism following simulated birth injury in rats. Neurourol. Urodyn. 32: 82-87.

2. Lou, LX. et al. 2009. Endoplasmic reticulum stress involved in heart and liver injury in iron-loaded rats. Clin. Exp. Pharmacol. Physiol. 36: 612-618.

Sequence Similarity

Belongs to the tubulin family.

Tissue Specificity

Ubiquitous.

Post-translational Modification

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Subcellular Location

Cytoplasm, Cytoskeleton, Microtubule.

UNIPROT

SwissProt: P68371 Human

SwissProt: Q13885 Human

SwissProt: Q9BVA1 Human

SwissProt: P68372 Mouse

SwissProt: Q7TMM9 Mouse

SwissProt: Q9CWF2 Mouse

SwissProt: P85108 Rat

SwissProt: Q3KRE8 Rat

SwissProt: Q6P9T8 Rat

Synonyms

bA506K6.1 antibody

Beta2 antibody

class II beta tubulin isotype antibody

class IIa beta-tubulin antibody

class IIb beta-tubulin antibody

Class IVb beta tubulin antibody

dJ40E16.7 antibody

DKFZp566F223 antibody

FLJ98847 antibody

M(beta)2 antibody

Expand

Images

  • Western blot analysis of beta II Tubulin on different lysates with Rabbit anti-beta II Tubulin antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 cell lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 3: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: Lane 1: 30 seconds; Lane 2-3: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta II Tubulin on different lysates with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/1,000 dilution.

    Lane 1: C2C12 cell lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)
    Lane 3: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: Lane 1: 30 seconds; Lane 2-3: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-beta II Tubulin antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-beta II Tubulin antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-beta II Tubulin antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-48" style="font-weight: bold;text-decoration: underline;">ET1609-48</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation