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HtrA2 / Omi Recombinant Rabbit Monoclonal Antibody [ST54-05]

Usd: 385

Specification

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Catalog# ET1609-73

HtrA2 / Omi Recombinant Rabbit Monoclonal Antibody [ST54-05]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HtrA2 / Omi Recombinant Rabbit Monoclonal Antibody [ST54-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human HtrA2 aa 80-188 / 458.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 49 kDa

Positive Control

HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, rat liver tissue, mouse liver tissue.

Conjugation

unconjugated

Clone Number

ST54-05

RRID

AB_3069882

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

The human homolog of the E. Coli htrA gene product, HtrA, is identified in osteoarthritic cartilage and is repressed in SV40-transformed fibroblast. The gene encoding HtrA protein is highly conserved among mammalian species and belongs to the serine protease family. The HtrA protein contains an IGF-binding domain and exhibits endoproteolytic activity, including autocatalytic cleavage. HtrA is a secreted protein that is expressed in heterologous systems. HtrA plays a role in the degradation of denatured proteins and cell growth regulation. Human HtrA2 (also designated Omi), a novel member of the HtrA serine protease family, is highly homologous to HtrA (also known as L56 and HtrA1). HtrA2 is a ubiquitously expressed nuclear protease that is capable of autoproteolysis. The HtrA2 protein exists as two polypeptides and as an alternatively spliced form called D-Omi, which is predominately expressed in the kidney, colon and thyroid. Due to a modified PDZ domain, D-Omi does not interact with the known partner of HtrA2, the Mxi2 protein. Like HtrA, HtrA2 is involved in the degradation aberrantly folded proteins during conditions of cellular stress, suggesting that it may possess a chaperone-like role under normal conditions.

Background References

1. Rami A et al. Translocation of the serine protease Omi/HtrA2 from mitochondria into the cytosol upon seizure-induced hippocampal injury in the neonatal rat brain. Neurochem Res 35:2199-207 (2010).

2. Sosna J et al. The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis. Cell Commun Signal 11:76 (2013).

Sequence Similarity

Belongs to the peptidase S1C family.

Tissue Specificity

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Post-translational Modification

Autoproteolytically activated.

Subcellular Location

Mitochondrion intermembrane space, Mitochondrion membrane.

UNIPROT

SwissProt: O43464 Human

SwissProt: Q9JIY5 Mouse

Unigene: 107325 Rat

Synonyms

High temperature requirement protein A2 antibody

HTRA 2 antibody

HtrA like serine protease antibody

HtrA serine peptidase 2 antibody

HtrA, E. coli, homolog of, 2 antibody

HtrA2 antibody

HTRA2_HUMAN antibody

mitochondrial antibody

Omi stress regulated endoprotease antibody

Omi stress-regulated endoprotease antibody

Expand

Images

  • Western blot analysis of HtrA2 / Omi on different lysates with Rabbit anti-HtrA2 / Omi antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 6: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 49 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 17 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HtrA2 / Omi on different lysates with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 5: Mouse liver tissue lysate (40 µg/Lane)
    Lane 6: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 49 kDa
    Observed band size: 38 kDa

    Exposure time: 17 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of HtrA2 / Omi on different lysates with Rabbit anti-HtrA2 / Omi antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-HtrA2 / Omi KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 49 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of HtrA2 / Omi on different lysates with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-HtrA2 / Omi KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 49 kDa
    Observed band size: 38 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-73) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HtrA2 / Omi antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HtrA2 / Omi antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HtrA2 / Omi antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-73" style="font-weight: bold;text-decoration: underline;">ET1609-73</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-73) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation