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Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08]

Usd: 385

Specification

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Catalog# ET1609-77

Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ACC1 aa 382-427 / 2346.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 266 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A549 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human kidney tissue, mouse placenta tissue.

Conjugation

unconjugated

Clone Number

ST53-08

RRID

AB_3069886

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB FC IF-Cell
human
Tested Tested
Not Recommended
Not Recommended
mouse
Tested Tested
Not Recommended
Not Recommended
rat
Tested Tested
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACCα (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCβ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCα is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the enzyme’s short-term regulatory mechanism. The gene encoding ACCα maps to human chromosome 17 and encodes a form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCβ is homologous to that of the ACCα, except for an additional peptide of about 150 amino acids at the N-terminus.

Background References

1. Tiwari M et al. Loss of Caspase-2-dependent Apoptosis Induces Autophagy after Mitochondrial Oxidative Stress in Primary Cultures of Young Adult Cortical Neurons. J Biol Chem 286:8493-506 (2011).

2. Chang NW et al. Fenofibrate exhibits a high potential to suppress the formation of squamous cell carcinoma in an oral-specific 4-nitroquinoline 1-oxide/arecoline mouse model. Biochim Biophys Acta 1812:558-64 (2011).

Tissue Specificity

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

Post-translational Modification

Phosphorylation on Ser-1263 is required for interaction with BRCA1.; Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.; The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q13085 Human

SwissProt: Q5SWU9 Mouse

SwissProt: P11497 Rat

Synonyms

ACAC antibody

ACACA antibody

ACACA_HUMAN antibody

ACC alpha antibody

ACC antibody

ACC-alpha antibody

ACC1 antibody

ACCA antibody

Acetyl CoA carboxylase 1 antibody

Acetyl CoA carboxylase alpha antibody

Expand

Images

  • Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: HEK-293 cell lysate (20 µg/Lane)<br />Lane 4: A549 cell lysate (20 µg/Lane)<br />Lane 5: C2C12 cell lysate (20 µg/Lane)<br />Lane 6: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 7: C6 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 266 kDa<br />Observed band size: 250/266 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: HEK-293 cell lysate (20 µg/Lane)
    Lane 4: A549 cell lysate (20 µg/Lane)
    Lane 5: C2C12 cell lysate (20 µg/Lane)
    Lane 6: RAW264.7 cell lysate (20 µg/Lane)
    Lane 7: C6 cell lysate (20 µg/Lane)

    Predicted band size: 266 kDa
    Observed band size: 250/266 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2-si NT cell lysate<br />Lane 2: HepG2-si ACC1 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 266 kDa<br />Observed band size: 250/266 kDa<br /><br />Exposure time: 25 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/1,000 dilution.

    Lane 1: HepG2-si NT cell lysate
    Lane 2: HepG2-si ACC1 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 266 kDa
    Observed band size: 250/266 kDa

    Exposure time: 25 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-77) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-77" style="font-weight: bold;text-decoration: underline;">ET1609-77</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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