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JAM-A Recombinant Rabbit Monoclonal Antibody [SC60-07]

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Specification

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Catalog# ET1610-90

JAM-A Recombinant Rabbit Monoclonal Antibody [SC60-07]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

JAM-A Recombinant Rabbit Monoclonal Antibody [SC60-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human F11R aa 1-100.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 33 kDa

Positive Control

HT-29 cell lysate, HUVEC cell lysate, TF-1 cell lysate, HeLa cell lysate, Human lung tissue lysate, human breast cancer tissue, human endometrium tissue.

Conjugation

unconjugated

Clone Number

SC60-07

RRID

AB_3069977

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:50-1:200

Target

Function

Junctional adhesion molecule A is a protein that in humans is encoded by the F11R gene. It has also been designated as CD321 (cluster of differentiation 321). Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor.

Background References

1. Sborov DW et al. A phase I trial of single-agent reolysin in patients with relapsed multiple myeloma. Clin Cancer Res 20:5946-55 (2014).

2. Manetti M et al. Differential expression of junctional adhesion molecules in different stages of systemic sclerosis. Arthritis Rheum 65:247-57 (2013).

Sequence Similarity

Belongs to the immunoglobulin superfamily.

Tissue Specificity

Expressed in endothelium, epithelium and leukocytes (at protein level).

Post-translational Modification

N-glycosylated.

Subcellular Location

Cell junction, Cell membrane.

UNIPROT

SwissProt: Q9Y624 Human

Synonyms

CD 321 antibody

CD321 antibody

CD321 antigen antibody

ESTM33 antibody

F11 receptor antibody

F11R antibody

JAM 1 antibody

JAM A antibody

JAM antibody

JAM-1 antibody

Expand

Images

  • Western blot analysis of JAM-A on different lysates with Rabbit anti-JAM-A antibody (<a href="/products/ET1610-90" style="font-weight: bold;text-decoration: underline;">ET1610-90</a>) at 1/2,000 dilution.<br /><br />Lane 1: HT-29 cell lysate (20 µg/Lane)<br />Lane 2: HUVEC cell lysate (20 µg/Lane)<br />Lane 3: TF-1 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (20 µg/Lane)<br />Lane 5: Human lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 33 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-90" style="font-weight: bold;text-decoration: underline;">ET1610-90</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of JAM-A on different lysates with Rabbit anti-JAM-A antibody (ET1610-90) at 1/2,000 dilution.

    Lane 1: HT-29 cell lysate (20 µg/Lane)
    Lane 2: HUVEC cell lysate (20 µg/Lane)
    Lane 3: TF-1 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (20 µg/Lane)
    Lane 5: Human lung tissue lysate (40 µg/Lane)

    Predicted band size: 33 kDa
    Observed band size: 37 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-JAM-A antibody (<a href="/products/ET1610-90" style="font-weight: bold;text-decoration: underline;">ET1610-90</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-90" style="font-weight: bold;text-decoration: underline;">ET1610-90</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-JAM-A antibody (ET1610-90) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-JAM-A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-90" style="font-weight: bold;text-decoration: underline;">ET1610-90</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-JAM-A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation