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hnRNP C1+C2 Recombinant Rabbit Monoclonal Antibody [SN0652]

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Specification

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Catalog# ET1611-2

hnRNP C1+C2 Recombinant Rabbit Monoclonal Antibody [SN0652]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

hnRNP C1+C2 Recombinant Rabbit Monoclonal Antibody [SN0652]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein of Human HNRNPC aa 60-190 / 306.

Species Reactivity

Human, Mouse, Rat, Zebrafish

Validated Applications

WB, IF-Tissue, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 34 kDa

Positive Control

MDA-MB-231 cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat brain tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, Hela, MCF-7, B16F1.

Conjugation

unconjugated

Clone Number

SN0652

RRID

AB_3069996

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:3,000-8,000

  • IF-Cell

  • 1:100-1:500

  • FC

  • 1:1,000

Target

Function

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and, in particular, the hnRNP A1 protein is one of the major pre-mRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, in-cluding hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the pre-mRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Background References

1. Juan WC et al. Identification of cis-Acting Elements and Splicing Factors Involved in the Regulation of BIM Pre-mRNA Splicing. PLoS One 9:e95210 (2014).

2. Cooper-Knock J et al. Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions. Brain 137:2040-51 (2014).

Sequence Similarity

Belongs to the RRM HNRPC family. RALY subfamily.

Post-translational Modification

Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.; Sumoylated. Sumoylation reduces affinity for mRNA.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P07910 Human

SwissProt: Q9Z204 Mouse

SwissProt: P17132 Rat

Synonyms

C1 antibody

C2 antibody

Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody

Heterogeneous nuclear ribonucleoprotein C antibody

Heterogeneous nuclear ribonucleoproteins C1/C2 antibody

HNRNP antibody

hnRNP C1 / hnRNP C2 antibody

hnRNP C1/C2 antibody

Hnrnpc antibody

HNRPC antibody

Expand

Images

  • Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/2,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate (10 µg/Lane)<br />Lane 2: HeLa cell lysate (10 µg/Lane)<br />Lane 3: HepG2 cell lysate (10 µg/Lane)<br />Lane 4: MCF7 cell lysate (10 µg/Lane)<br />Lane 5: HL-60 cell lysate (10 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 34 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: Lane 1-5: 10 seconds; Lane 6: 46 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/2,000 dilution.

    Lane 1: MDA-MB-231 cell lysate (10 µg/Lane)
    Lane 2: HeLa cell lysate (10 µg/Lane)
    Lane 3: HepG2 cell lysate (10 µg/Lane)
    Lane 4: MCF7 cell lysate (10 µg/Lane)
    Lane 5: HL-60 cell lysate (10 µg/Lane)
    Lane 6: Mouse brain tissue lysate (20 µg/Lane)

    Predicted band size: 34 kDa
    Observed band size: 43 kDa

    Exposure time: Lane 1-5: 10 seconds; Lane 6: 46 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 3: PC-12 cell lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 5: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 34 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 3: PC-12 cell lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (40 µg/Lane)
    Lane 5: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 34 kDa
    Observed band size: 43 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-2) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-2) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-2) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining hnRNP C1+C2 in B16F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining hnRNP C1+C2 in B16F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Flow cytometric analysis of HeLa cells labeling hnRNP C1+C2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1611-2" style="font-weight: bold;text-decoration: underline;">ET1611-2</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling hnRNP C1+C2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1611-2, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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