NSD3 Recombinant Rabbit Monoclonal Antibody [SN07-11]
Catalog# ET1611-39
NSD3 Recombinant Rabbit Monoclonal Antibody [SN07-11]
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WB
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FC
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NSD3 Recombinant Rabbit Monoclonal Antibody [SN07-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human NSD3 aa 1-50 / 1,437.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC, IHC-P
Molecular Weight
Predicted band size: 162 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat eyeball tissue lysate, human small intestine tissue, human lymph nodes tissue, Hela.
Conjugation
unconjugated
Clone Number
SN07-11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:10,000
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FC
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1:50-1:100
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IHC-P
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1:50-1:400
Target
Function
The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs.
Background References
1. Hendriks I.A., et al. 2015. SUMO-2 orchestrates chromatin modifiers in response to DNA damage. Cell Rep. 10:1778-1791.
2. Rahman S et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011).
Sequence Similarity
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
Tissue Specificity
Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
Subcellular Location
Nucleus, Chromosome.
Synonyms
DKFZp667H044 antibody
FLJ20353 antibody
Histone lysine N methyltransferase NSD3 antibody
Histone-lysine N-methyltransferase NSD3 antibody
MGC126766 antibody
MGC142029 antibody
NSD 3 antibody
NSD3_HUMAN antibody
Nuclear set domain containing 3 antibody
Nuclear SET domain containing protein 3 antibody
ExpandImages
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Western blot analysis of NSD3 on different lysates with Rabbit anti-NSD3 antibody (ET1611-39) at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat eyeball tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 162 kDa
Observed band size: 162 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-39) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NSD3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-NSD3 antibody (ET1611-39) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of NSD3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"