NOX2/gp91phox Recombinant Rabbit Monoclonal Antibody [SN07-16] (ET1611-44)
Catalog# ET1611-44
Rabbit Monoclonal to NOX2/gp91phox
-
WB
-
Human
-
Mouse
-
Rat
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: MCF7 cell lysate (15 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 65 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-44) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NOX2/gp91phox Recombinant Rabbit Monoclonal Antibody [SN07-16] (ET1611-44)
Immunogen
Synthetic peptide within human nox2 aa 131-180 / 570.
Host
Rabbit
Positive Control
HepG2 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, rat spleen tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN07-16
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 65 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NOX2/gp91phox
SYNONYMS
AMCBX2 antibody;CGD antibody;CGD91-phox antibody;CY24B_HUMAN antibody;CYBB antibody;Cytochrome b 245, beta polypeptide antibody;Cytochrome b(558) beta chain antibody;Cytochrome b(558) subunit beta antibody;Cytochrome b-245 heavy chain antibody;Cytochrome b558 subunit beta antibody;GP91 PHOX antibody;gp91-1 antibody;gp91-phox antibody;GP91PHOX antibody;Heme-binding membrane glycoprotein gp91phox antibody;NADPH oxidase 2 antibody;Neutrophil cytochrome b 91 kDa polypeptide antibody;NOX2 antibody;p22 phagocyte B-cytochrome antibody;P91 PHOX antibody;p91-PHOX antibody;Superoxide-generating NADPH oxidase heavy chain subunit antibody
TISSUE SPECIFICITY
Detected in neutrophils (at protein level).
POST-TRANSLATIONAL MODIFICATION
Glycosylated.; Phosphorylated on Ser and Thr residues.; Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.
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