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Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [SN72-09]

Usd: 385

Specification

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Catalog# ET1611-48

Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [SN72-09]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

  • IP

  • ChIP

  • CUT&Tag-seq

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [SN72-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Histone H2B aa 1-49/126.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP, ChIP, CUT&Tag-seq

Molecular Weight

Predicted band size: 14 kDa

Positive Control

SW480, MCF-7, Hela, human tonsil tissue, human kidney tissue, human colon tissue, mouse colon tissue, human colon cancer tissue, mouse kidney tissue, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

SN72-09

RRID

AB_3070027

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

  • ChIP

  • Use 0.5~2 μg for 25 μg of chromatin.

Target

Function

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Background References

1. Zhang B et al. An integrated peptide-antigen microarray on plasmonic gold films for sensitive human antibody profiling. PLoS One 8:e71043 (2013).

2. Price JV et al. On silico peptide microarrays for high-resolution mapping of antibody epitopes and diverse protein-protein interactions. Nat Med : (2012).

Sequence Similarity

Belongs to the histone H2B family.

Post-translational Modification

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.; Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.; GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Subcellular Location

Chromosome, Nucleosome core, Nucleus.

UNIPROT

SwissProt: O60814 Human

SwissProt: P06899 Human

SwissProt: P23527 Human

SwissProt: P33778 Human

SwissProt: P57053 Human

SwissProt: P58876 Human

SwissProt: P62807 Human

SwissProt: Q16778 Human

SwissProt: Q5QNW6 Human

SwissProt: Q8N257 Human

SwissProt: Q93079 Human

SwissProt: Q99877 Human

SwissProt: Q99879 Human

SwissProt: Q99880 Human

Entrez Gene: 64647 Mouse

Entrez Gene: Q00729 Rat

Synonyms

H2B 1A antibody

H2B antibody

H2B histone family antibody

H2B2f antibody

H2B2F_HUMAN antibody

H2Ba antibody

H2Bf antibody

HIST2H2BF antibody

histone H2B antibody

histone H2B type 1 antibody

Expand

Images

  • ICC staining Histone H2B(acetyl K20) (1/500) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Histone H2B(acetyl K20) (1/500) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Histone H2B(acetyl K20) (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Histone H2B(acetyl K20) (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Histone H2B(acetyl K20) (1/200) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Histone H2B(acetyl K20) (1/200) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2B (acetyl K20) (<a href="/products/ET1611-48" style="font-weight: bold;text-decoration: underline;">ET1611-48</a>) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    ☑ Cell treatment (CT)

    Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2B (acetyl K20) (ET1611-48) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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