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Phospho-Cdk2 (Y15) Recombinant Rabbit Monoclonal Antibody [SN72-04]

Usd: 385

Specification

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Catalog# ET1611-52

Phospho-Cdk2 (Y15) Recombinant Rabbit Monoclonal Antibody [SN72-04]

Application

  • WB

  • IHC-P

  • IP

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-Cdk2 (Y15) Recombinant Rabbit Monoclonal Antibody [SN72-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP, IF-Tissue

Molecular Weight

Predicted band size: 34 kDa

Positive Control

Hela cell lysate, NIH/3T3 cell lysate, mouse spleen tissue lysate, human breast carcinoma tissue, mouse small intestine tissue.

Conjugation

unconjugated

Clone Number

SN72-04

RRID

AB_3070032

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IP

  • Use at an assay dependent concentration.

  • IF-Tissue

  • 1:50-1:200

Target

Function

In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE.

Background References

1. Gao K et al. HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma. Biochem Biophys Res Commun 458:849-55 (2015).

2. Wang G et al. Synergistic antitumor interactions between MK-1775 and panobinostat in preclinical models of pancreatic cancer. Cancer Lett 356:656-68 (2015).

Sequence Similarity

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Post-translational Modification

Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.; Nitrosylated after treatment with nitric oxide (DETA-NO).

Subcellular Location

Cytoplasm, Cytoskeleton, Endosome, Nucleus.

UNIPROT

SwissProt: P24941 Human

SwissProt: P97377 Mouse

SwissProt: Q63699 Rat

Synonyms

Cdc2 related protein kinase antibody

cdc2-related protein kinase antibody

CDC28 antibody

CDC2A antibody

Cdk 2 antibody

CDK1 antibody

CDK2 antibody

CDK2_HUMAN antibody

CDKN2 antibody

Cell devision kinase 2 antibody

Expand

Images

  • Western blot analysis of Phospho-Cdk2 (Y15) on different lysates with Rabbit anti-Phospho-Cdk2 (Y15) antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate (10 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (10 µg/Lane)<br />Lane 3: Mouse spleen tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 34 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 1 minute;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-Cdk2 (Y15) on different lysates with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution.

    Lane 1: Hela cell lysate (10 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
    Lane 3: Mouse spleen tissue lysate (20 µg/Lane)

    Predicted band size: 34 kDa
    Observed band size: 35 kDa

    Exposure time: 1 minute;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Cdk2(Y15) on Hela cell lysates.<br /><br />Lane 1: Hela cells, whole cell lysate, 10ug/lane<br />Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane<br /><br />All lanes :<br />Anti-Phospho-Cdk2(Y15) antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/500 dilution. Anti-Cdk2 antibody (<a href="/products/ET1602-6" style="font-weight: bold;text-decoration: underline;">ET1602-6</a>) at 1/500 dilution. Anti-GAPDH antibody (<a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/200,000 dilution.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 34 kDa<br /><br />Blocking and diluting buffer: 5% BSA.<br /><br />Exposure time: 30 seconds

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Cdk2(Y15) on Hela cell lysates.

    Lane 1: Hela cells, whole cell lysate, 10ug/lane
    Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane

    All lanes :
    Anti-Phospho-Cdk2(Y15) antibody (ET1611-52) at 1/500 dilution. Anti-Cdk2 antibody (ET1602-6) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

    Blocking and diluting buffer: 5% BSA.

    Exposure time: 30 seconds

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-52" style="font-weight: bold;text-decoration: underline;">ET1611-52</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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