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Cyclin B2 Recombinant Rabbit Monoclonal Antibody [SD2045]

Usd: 385

Specification

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Catalog# ET1612-21

Cyclin B2 Recombinant Rabbit Monoclonal Antibody [SD2045]

Application

  • WB

  • IF-Cell

  • IP

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cyclin B2 Recombinant Rabbit Monoclonal Antibody [SD2045]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cyclin B2 aa 1-102 / 398.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, IP

Molecular Weight

Predicted band size: 45 kDa

Positive Control

A549 cell lysate, PC-12 cell lysate, rat testis tissue lysate, PC-12.

Conjugation

unconjugated

Clone Number

SD2045

RRID

AB_3070094

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

Target

Function

In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Background References

1. Davies C et al. Murine norovirus replication induces G0/G1 cell cycle arrest in asynchronously growing cells. J Virol 89:6057-66 (2015).

2. Dupré A et al. Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in Xenopus oocytes. Nat Commun 5:3318 (2014).

Sequence Similarity

Belongs to the cyclin family. Cyclin AB subfamily.

Subcellular Location

Centrosome, microtubule cytoskeleton, cytosol, nucleus, cyclin-dependent protein kinase holoenzyme complex, cytoplasm, host cell nucleus, membrane.

UNIPROT

SwissProt: O95067 Human

Entrez Gene: 363088 Rat

Synonyms

ccnb2 antibody

CCNB2_HUMAN antibody

CycB2 antibody

Cyclin B2 antibody

G2 mitotic specific cyclin B2 antibody

G2/mitotic specific cyclin B2 antibody

G2/mitotic-specific cyclin-B2 antibody

HsT17299 antibody

MGC108931 antibody

MGC140694 antibody

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cyclin B2 on different lysates with Rabbit anti-Cyclin B2 antibody (<a href="/products/ET1612-21" style="font-weight: bold;text-decoration: underline;">ET1612-21</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD Cyclin B2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-21" style="font-weight: bold;text-decoration: underline;">ET1612-21</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cyclin B2 on different lysates with Rabbit anti-Cyclin B2 antibody (ET1612-21) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD Cyclin B2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cyclin B2 on different lysates with Rabbit anti-Cyclin B2 antibody (<a href="/products/ET1612-21" style="font-weight: bold;text-decoration: underline;">ET1612-21</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: PC-12 cell lysate (20 µg/Lane)<br />Lane 3: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-21" style="font-weight: bold;text-decoration: underline;">ET1612-21</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cyclin B2 on different lysates with Rabbit anti-Cyclin B2 antibody (ET1612-21) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: PC-12 cell lysate (20 µg/Lane)
    Lane 3: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Cyclin B2 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1612-21" style="font-weight: bold;text-decoration: underline;">ET1612-21</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Cyclin B2 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation