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pro Caspase-7 Recombinant Rabbit Monoclonal Antibody [SD20-57]

Usd: 385

Specification

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Catalog# ET1612-28

pro Caspase-7 Recombinant Rabbit Monoclonal Antibody [SD20-57]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

pro Caspase-7 Recombinant Rabbit Monoclonal Antibody [SD20-57]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Caspase 7 aa 1-50 / 303.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

34 kDa

Positive Control

Hela, HepG2, human liver tissue, human kidney tissue,human lung lysates.

Conjugation

unconjugated

Clone Number

SD20-57

RRID

AB_2940744

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

A unique family of Cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

Background References

1. Wang, Z.H. et al. 2015. Cytotoxic effect of s-petasin and iso-s-petasin on the proliferation of human prostate cancer cells. Anticancer research. 35: 191-9.

2. Zhen, J. et al. 2014. AIM2 mediates inflammation-associated renal damage in hepatitis B virus-associated glomerulonephritis by regulating caspase-1, IL-1β, and IL-18. Mediators of inflammation. 2014: 190860.

Sequence Similarity

Belongs to the peptidase C14A family.

Tissue Specificity

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Post-translational Modification

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P55210 Human

Synonyms

Caspase7

apoptosis-related cysteine peptidase antibody

Apoptotic protease Mch-3 antibody

CASP-7 antibody

CASP7 antibody

CASP7_HUMAN antibody

Caspase-7 subunit p11 antibody

Caspase-7 antibody

Caspase 7 antibody

Caspase7 antibody

Expand

Images

  • Western blot analysis of pro Caspase-7 on human lung lysates with Rabbit anti-pro Caspase-7 antibody (<a href="/products/ET1612-28" style="font-weight: bold;text-decoration: underline;">ET1612-28</a>) at 1/1000 dilution.<br /><br />Lysates at 20 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 35 kDa<br /><br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-28" style="font-weight: bold;text-decoration: underline;">ET1612-28</a>) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of pro Caspase-7 on human lung lysates with Rabbit anti-pro Caspase-7 antibody (ET1612-28) at 1/1000 dilution.

    Lysates at 20 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 35 kDa


    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-28) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining pro Caspase 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining pro Caspase 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining pro Caspase 7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining pro Caspase 7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation