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Cyclin D3 Recombinant Rabbit Monoclonal Antibody [SD20-43]

Usd: 385

Specification

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Catalog# ET1612-4

Cyclin D3 Recombinant Rabbit Monoclonal Antibody [SD20-43]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cyclin D3 Recombinant Rabbit Monoclonal Antibody [SD20-43]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cyclin D3 aa 162-292 / 292.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 33 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, A20 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate.

Conjugation

unconjugated

Clone Number

SD20-43

RRID

AB_3070113

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

Background References

1. Misiewicz-Krzeminska I et al. Post-transcriptional Modifications Contribute to the Upregulation of Cyclin D2 in Multiple Myeloma. Clin Cancer Res 22:207-17 (2016).

2. Brodowska K et al. Effects of metformin on retinoblastoma growth in vitro and in vivo. Int J Oncol 45:2311-24 (2014).

Sequence Similarity

Belongs to the cyclin family. Cyclin D subfamily.

Post-translational Modification

Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Subcellular Location

Nucleus, Cytoplasm, Membrane.

UNIPROT

SwissProt: P30281 Human

SwissProt: P30282 Mouse

SwissProt: P48961 Rat

Synonyms

CCND 3 antibody

Ccnd3 antibody

CCND3_HUMAN antibody

CyclinD3 antibody

D3 type cyclin antibody

G1 S specific cyclin D3 antibody

G1/S specific cyclin D3 antibody

G1/S-specific cyclin-D3 antibody

Images

  • Western blot analysis of Cyclin D3 on different lysates with Rabbit anti-Cyclin D3 antibody (<a href="/products/ET1612-4" style="font-weight: bold;text-decoration: underline;">ET1612-4</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: A20 cell lysate<br />Lane 5: C2C12 cell lysate<br />Lane 6: PC-12 cell lysate<br />Lane 7: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-4" style="font-weight: bold;text-decoration: underline;">ET1612-4</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cyclin D3 on different lysates with Rabbit anti-Cyclin D3 antibody (ET1612-4) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: A20 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation