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Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814]

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Specification

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Catalog# ET1612-53

Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Stromal aa 561-685 / 685.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 77 kDa

Positive Control

SH-SY5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Human lung tissue lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, human thyroid tissue, mouse skeletal muscle tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

SD0814

RRID

AB_3070127

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:500

Target

Function

Ca2+ influx is essential for a variety of cellular functions including, secretion and transcription. Stromal interaction molecule 1 (Stim1) is a ubiquitously expressed cell surface transmembrane glycoprotein that plays a role in medi-ating Ca2+ influx following the depletion of intracellular Ca2+ stores. Stim1 functions in the endoplasmic reticulum (ER) where it acts as a Ca2+ sensor via its EF-hand domain which causes large conformational changes. When Ca2+ levels drop, Stim1 translocates from the ER to the plasma membrane, where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/Orai1. Stim2 is a potent inhibitor of Stim1-mediated store-operated calcium (SOC) entry. Stim1 is implicated in tumor growth suppression and stromal-hematopoietic cell interactions.

Background References

1. Li Y et al. High expression of STIM1 in the early stages of diffuse axonal injury. Brain Res 1495:95-102 (2013).

2. Liu, H. et al. Calcium entry via ORAI1 regulates glioblastoma cell proliferation and apoptosis. Exp. Mol. Pathol.. 91: 753-760 (2011).

Tissue Specificity

Ubiquitously expressed in various human primary cells and tumor cell lines.

Post-translational Modification

Glycosylation is required for cell surface expression.; Phosphorylated predominantly on Ser residues.

Subcellular Location

Cell membrane, Endoplasmic reticulum membrane, Cytoplasm, Sarcoplasmic reticulum.

UNIPROT

SwissProt: Q13586 Human

SwissProt: P70302 Mouse

SwissProt: P84903 Rat

Synonyms

D11S4896E antibody

GOK antibody

OTTHUMP00000164512 antibody

OTTHUMP00000229140 antibody

OTTHUMP00000230742 antibody

SIM antibody

STIM 1 antibody

STIM1 antibody

Stim1 stromal interaction molecule 1 antibody

STIM1_HUMAN antibody

Expand

Images

  • Western blot analysis of Stromal interaction molecule 1 on different lysates with Rabbit anti-Stromal interaction molecule 1 antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 3: PC-12 cell lysate (20 µg/Lane)<br />Lane 4: Human lung tissue lysate (40 µg/Lane)<br />Lane 5: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 6: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 77 kDa<br />Observed band size: 77 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Stromal interaction molecule 1 on different lysates with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 3: PC-12 cell lysate (20 µg/Lane)
    Lane 4: Human lung tissue lysate (40 µg/Lane)
    Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 6: Rat thymus tissue lysate (40 µg/Lane)

    Predicted band size: 77 kDa
    Observed band size: 77 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Stromal interaction molecule 1 antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Stromal interaction molecule 1 antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-53" style="font-weight: bold;text-decoration: underline;">ET1612-53</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation