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PAX8 Recombinant Rabbit Monoclonal Antibody [SD0832]

Usd: 385

Specification

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Catalog# ET1612-61

PAX8 Recombinant Rabbit Monoclonal Antibody [SD0832]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PAX8 Recombinant Rabbit Monoclonal Antibody [SD0832]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human PAX8 aa 260-450 / 450.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Target Molecular Weight

Predicted band size: 48 kDa

Positive Control

SKOV-3 cell lysates, SKOV-3, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

SD0832

RRID

AB_3070136

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50

  • IHC-P

  • 1:200

Target

Function

The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. Pax-8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. Pax-8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases Pax-8 mRNA levels and Pax-8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the Pax-8 gene develop thyroid dysgenesis. The Pax-8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The Pax-8 isoforms display different DNA binding capacities and are thought to be functionally distinct. The gene which encodes Pax-8 maps to human chromosome 2q12-q14.

Background References

1. Sicking EM et al. Subtotal ablation of parietal epithelial cells induces crescent formation. J Am Soc Nephrol 23:629-40 (2012).

2. Borgogni E et al. Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells. Endocrinology 149:3626-34 (2008).

Tissue Specificity

Expressed in the excretory system, thyroid gland and Wilms tumors.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q06710 Human

SwissProt: Q00288 Mouse

SwissProt: P51974 Rat

Synonyms

OTTHUMP00000158659 antibody

OTTHUMP00000158660 antibody

OTTHUMP00000203723 antibody

OTTHUMP00000203724 antibody

Paired box 8 antibody

Paired box gene 8 antibody

paired box homeotic gene 8 antibody

Paired box protein Pax 8 antibody

Paired box protein Pax-8 antibody

Paired domain gene 8 antibody

Expand

Images

  • Western blot analysis of PAX8 on different lysates with Rabbit anti-PAX8 antibody (<a href="/products/ET1612-61" style="font-weight: bold;text-decoration: underline;">ET1612-61</a>) at 1/5,000 dilution.<br /><br />Lane 1: SK-OV-3 (Human ovarian cancer cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 110 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1612-61" style="font-weight: bold;text-decoration: underline;">ET1612-61</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa

    Western blot analysis of PAX8 on different lysates with Rabbit anti-PAX8 antibody (ET1612-61) at 1/5,000 dilution.

    Lane 1: SK-OV-3 (Human ovarian cancer cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 110 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1612-61, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PAX8 antibody (<a href="/products/ET1612-61" style="font-weight: bold;text-decoration: underline;">ET1612-61</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-61" style="font-weight: bold;text-decoration: underline;">ET1612-61</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PAX8 antibody (ET1612-61) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PAX8 antibody (<a href="/products/ET1612-61" style="font-weight: bold;text-decoration: underline;">ET1612-61</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-61" style="font-weight: bold;text-decoration: underline;">ET1612-61</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PAX8 antibody (ET1612-61) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"