Phospho-CDC37 (S13) Recombinant Rabbit Monoclonal Antibody [SD08-50]
Catalog# ET1612-74
Phospho-CDC37 (S13) Recombinant Rabbit Monoclonal Antibody [SD08-50]
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WB
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Phospho-CDC37 (S13) Recombinant Rabbit Monoclonal Antibody [SD08-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser13 of human CDC37.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, PC-12 cell lysate, HEK-293 cell lysate treated with λpp for 1 hour, C2C12 cell lysate treated with λpp for 1 hour.
Conjugation
unconjugated
Clone Number
SD08-50
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IP
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Use at an assay dependent concentration.
Target
Function
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
Background References
1. Synoradzki K & Bieganowski P Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast. Biochim Biophys Acta 1853:445-52 (2015).
2. Xavier CP et al. Phosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration. Sci Rep 2:241 (2012).
Sequence Similarity
Belongs to the CDC37 family.
Post-translational Modification
Constitutively sumoylated by UBE2I.
Subcellular Location
Cytoplasm.
Synonyms
CDC 37 antibody
Cdc37 antibody
CDC37 cell division cycle 37 homolog antibody
CDC37 cell division cycle 37 S cerevisiae homolog antibody
CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
Cdc37 homolog antibody
CDC37 protein antibody
CDC37_HUMAN antibody
CDC37A antibody
cell division cycle 37 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-CDC37 (S13) on different lysates with Rabbit anti-Phospho-CDC37 (S13) antibody (ET1612-74) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: C2C12 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: HEK-293 cell lysate, treated with λpp for 1 hour
Lane 6: C2C12 cell lysate, treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-74) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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