SOX11 Recombinant Rabbit Monoclonal Antibody [SD203-08]
Catalog# ET1612-76
SOX11 Recombinant Rabbit Monoclonal Antibody [SD203-08]
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WB
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IF-Cell
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IF-Tissue
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IP
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SOX11 Recombinant Rabbit Monoclonal Antibody [SD203-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SOX11 aa 1-50 / 441.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IP, IHC-P
Molecular Weight
47 kDa
Positive Control
Human brain tissue lysates, rat spinal cord tissue lysates, SH-SY5Y, N2A, human mantle cell lymphoma tissue.
Conjugation
unconjugated
Clone Number
SD203-08
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:200
Target
Function
The Sox family are comprised of members that are related to the mammalian sex determining gene SRY. The Sox family of genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been conserved during evolution and play key roles during animal development. Some are involved in human diseases, including sex reversal. Sox-11 (SRY (sex determining region Y)-box 11) is a 441 amino acid nuclear protein expressed mainly in the nervous system. Containing an HMG box DNA-binding domain, Sox-11 may play a critical role in the developing nervous system.
Background References
1. Murugan, S. et al. 2012. WT1 and Sox11 regulate synergistically the promoter of the Wnt4 gene that encodes a critical signal for nephrogenesis. Exp. Cell Res.. 318: 1134-1145.
2. Jankowski, MP. et al. 2009. Sox11 transcription factor modulates peripheral nerve regeneration in adult mice. Brain Res.. 1256: 43-54.
Tissue Specificity
Expressed primarily in the brain and heart, with low expression in the kidney, pancreas and muscle.
Subcellular Location
Nucleus.
Synonyms
MRD27 antibody
SOX11 antibody
SOX11_HUMAN antibody
SRY (sex determining region Y) box 11 antibody
SRY related HMG box gene 11 antibody
SRY-box 11 antibody
Transcription factor SOX-11 antibody
Images
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Western blot analysis of SOX11 on human brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of SOX11 on rat spinal cord tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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ICC staining of SOX11 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of SOX11 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma tissue with Rabbit anti-SOX11 antibody (ET1612-76) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-76) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"