Hsp20 Recombinant Rabbit Monoclonal Antibody [SD086-03]
Catalog# ET1612-81
Hsp20 Recombinant Rabbit Monoclonal Antibody [SD086-03]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Hsp20 Recombinant Rabbit Monoclonal Antibody [SD086-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Hsp20 aa 81-130 / 160.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 17 kDa
Positive Control
Human skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysates, mouse skeletal muscle tissue, mouse heart tissue, Hela.
Conjugation
unconjugated
Clone Number
SD086-03
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:500
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FC
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1:50-1:100
Target
Function
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. HSP 20 occurs in two complex sizes, dimers and multimers. It is related to stress proteins and occurs most abundantly in skeletal muscle and heart. HSP 20 is considerably shorter at the C-terminus and less polar than other small heat shock proteins.
Background References
1. Hedegaard ER et al. Mechanisms involved in increased sensitivity to adenosine A(2A) receptor activation and hypoxia-induced vasodilatation in porcine coronary arteries. Eur J Pharmacol 723:216-26 (2014).
2. Kirui DK et al. Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors. PLoS One 9:e86489 (2014).
Sequence Similarity
Belongs to the small heat shock protein (HSP20) family.
Post-translational Modification
The N-terminus is blocked.; Phosphorylated at Ser-16 by PKA and probably PKD1K; required to protect cardiomyocytes from apoptosis.
Subcellular Location
Cytoplasm, Secreted, Nucleus.
Synonyms
epididymis luminal protein 55 antibody
FLJ32389 antibody
Heat shock 20 kDa like protein p20 antibody
Heat shock 20 kDa-like protein p20 antibody
Heat shock protein alpha crystallin related B6 antibody
Heat shock protein beta 6 antibody
Heat shock protein beta-6 antibody
Heat shock protein, 20-KD antibody
Heat-shock 27-KD protein 6 antibody
HEL55 antibody
ExpandImages
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Western blot analysis of Hsp20 on different lysates with Rabbit anti-Hsp20 antibody (ET1612-81) at 1/500 dilution.
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1612-81, 1/500 in 5% BSA, room temperature for 2 hours
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1:200,000, 1 hour at room temperature
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Western blot analysis of Hsp20 on rat skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Hsp20 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-81, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"