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IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]

Usd: 385

Specification

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Catalog# ET1701-39

IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human IL-1 beta aa 117-269.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 31 kDa

Positive Control

THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for last 3 hours cell lysate, THP-1, human kidney tissue using anti-商品名 antibody. The section was pre-treated using heat mediated antigen retrieval.

Conjugation

unconjugated

Clone Number

JJ087-3

RRID

AB_3070207

同源性数据

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Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:500

  • IHC-P

  • 1:50

Target

Function

Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1β for production of the anti- pathogen IFN-γ. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.

Background References

1. Qiao J et al. Autologous platelet rich plasma injection can be effective in the management of osteoarthritis of the knee: impact on IL-1 beta, TNF-alpha, hs-CRP. J Orthop Surg Res. 2024 Oct

2. Vicens-Artés S et al. Effect of MP-AzeFlu in IL-1 beta-induced IL-6 and proinflammatory cytokines. Immunol Res. 2023 Jun

Sequence Similarity

Belongs to the IL-1 family.

Tissue Specificity

Expressed in activated monocytes/macrophages (at protein level).

Post-translational Modification

Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Subcellular Location

Cytoplasm, Lysosome, extracellular exosome, Secreted.

UNIPROT

SwissProt: P01584 Human

Synonyms

Catabolin antibody

H1 antibody

IL 1 antibody

IL 1 beta antibody

IL-1 beta antibody

IL1 BETA antibody

IL1B antibody

IL1B_HUMAN antibody

IL1F2 antibody

Interleukin 1 beta antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for last 3 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 35/30 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/5,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for last 3 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 35/30 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-39) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of THP-1 cells untreated with or without TPA(80nM overnight) then LPS(100ng/mL 6h)+BFA(300ng/mL 3h) labeling IL-1 beta with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/500 dilution.<br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of THP-1 cells untreated with or without TPA(80nM overnight) then LPS(100ng/mL 6h)+BFA(300ng/mL 3h) labeling IL-1 beta with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/500 dilution.
    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human Kidney tissue with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human Kidney tissue with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-39) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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