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PD-L1 Recombinant Rabbit Monoclonal Antibody [JJ08-95]

Usd: 205

Specification

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Catalog# ET1701-41

PD-L1 Recombinant Rabbit Monoclonal Antibody [JJ08-95]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PD-L1 Recombinant Rabbit Monoclonal Antibody [JJ08-95]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PD-L1 aa 191-240 / 290.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 33 kDa

Positive Control

MDA-MB-231 cell lysate, A375 cell lysate, U-87 MG cell lysate, THP-1 cell lysate, human non-small cell lung cancer tissue.

Conjugation

unconjugated

Clone Number

JJ08-95

RRID

AB_3073462

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:50-1:200

Target

Function

Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells, and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. Pdcd-1L1 (programmed cell death ligand-1), also known as B7-H1 or PD-L1, is 290 amino acid type I transmembrane protein which is 20% and 15% identical to B7-1 and B7-2, respectively. Pdcd-1L2 has immunoglobulin V-like and C-like domains and a 30 amino acid cytoplasmic tail. It does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of Pdcd-1L1 co-stimulation. The gene which encodes Pdcd-1L1 maps to human chromosome 9p24. Pdcd-1L2 (programmed cell death ligand-2) is a 73 amino acid protein which contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The gene which encodes Pdcd-1L2 maps to human chromosome 9p24.2. The constitutive expression of Pdcd-1L1 and Pdcd-1L2 on paren-chymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.

Background References

1. Jurado J.O., et al. 2008. Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis. J. Immunol. 181:116-125.

2. Boorjian S.A., et al. 2008. T-cell coregulatory molecule expression in urothelial cell carcinoma: clinicopathologic correlations and association with survival. Clin. Cancer Res. 14:4800-4808.

Sequence Similarity

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Tissue Specificity

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Post-translational Modification

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.

Subcellular Location

Cell membrane, Early endosome membrane, Recycling endosome membrane, Nucleus.

UNIPROT

SwissProt: Q9NZQ7 Human

Synonyms

B7 H antibody

B7 H1 antibody

B7 homolog 1 antibody

B7-H1 antibody

B7H antibody

B7H1 antibody

CD 274 antibody

CD274 antibody

CD274 antigen antibody

CD274 molecule antibody

Expand

Images

  • Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (<a href="/products/ET1701-41" style="font-weight: bold;text-decoration: underline;">ET1701-41</a>) at 1/5,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate<br />Lane 2: A375 cell lysate<br />Lane 3: U-87 MG cell lysate<br />Lane 4: THP-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-41" style="font-weight: bold;text-decoration: underline;">ET1701-41</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (ET1701-41) at 1/5,000 dilution.

    Lane 1: MDA-MB-231 cell lysate
    Lane 2: A375 cell lysate
    Lane 3: U-87 MG cell lysate
    Lane 4: THP-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer tissue using anti-PD-L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1701-41" style="font-weight: bold;text-decoration: underline;">ET1701-41</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer tissue using anti-PD-L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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