FOXO4 Recombinant Rabbit Monoclonal Antibody [JJ09-11]
Catalog# ET1701-67
FOXO4 Recombinant Rabbit Monoclonal Antibody [JJ09-11]
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WB
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
FOXO4 Recombinant Rabbit Monoclonal Antibody [JJ09-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human FOXO4 aa 456-505 / 505.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IF-Tissue, IHC-P, FC, IP
Molecular Weight
Predicted band size: 54 kDa
Positive Control
293T, Hela, HepG2, human placenta tissue, 293.
Conjugation
unconjugated
Clone Number
JJ09-11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
Target
Function
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Background References
1. Liu X et al. MicroRNA-499-5p promotes cellular invasion and tumor metastasis in colorectal cancer by targeting FOXO4 and PDCD4. Carcinogenesis 32:1798-805 (2011).
2. Iwata J et al. Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells. J Biol Chem 285:4975-82 (2010).
Tissue Specificity
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
Post-translational Modification
Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.; Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK.; Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
AFX antibody
AFX1 antibody
Afxh antibody
ALL1-fused gene from X chromosome antibody
Fork head domain transcription factor AFX1 antibody
Forkhead box O4 antibody
Forkhead box protein O4 antibody
FOXO 4 antibody
Foxo4 antibody
FOXO4_HUMAN antibody
ExpandImages
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Western blot analysis of FOXO4 on different lysates with Rabbit anti-FOXO4 antibody (ET1701-67) at 1/500 dilution.
Lane 1: HeLa cell lysate
Lane 2: Mouse heart tissue lysate
Lysates/proteins at 10 µg/Lane1 and 20 ug/Lane2.
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-67) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-FOXO4 antibody (ET1701-67) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-67) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of 293 cells labeling FOXO4.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-67, 1:1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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