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Occludin Recombinant Rabbit Monoclonal Antibody [JJ091-08] (ET1701-76)

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Catalog# ET1701-76

Rabbit Monoclonal to Occludin

Tested for

  • WB

  • IP

  • FC

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Occludin Recombinant Rabbit Monoclonal Antibody [JJ091-08] (ET1701-76)

Immunogen

Synthetic peptide within n-terminal human occludin.

Host

Rabbit

Positive Control

MCF-7 cell lysate, Mouse smooth muscle tissue lysate, Caco-2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ091-08

RRID

AB_3070241

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

  • IF-Cell

  • 1:500

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 59 kDa

Isotype

IgG

TARGET

UNIPROT #

Q16625

PROTEIN NAME

Occludin

SYNONYMS

BLCPMG antibody;FLJ08163 antibody;FLJ18079 antibody;FLJ77961 antibody;FLJ94056 antibody;MGC34277 antibody;Occludin antibody;Ocln antibody;OCLN_HUMAN antibody;Tight junction protein occludin antibody

SEQUENCE SIMILARITIES

Belongs to the ELL/occludin family.

TISSUE SPECIFICITY

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

POST-TRANSLATIONAL MODIFICATION

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

SUBCELLULAR LOCATION

Cell membrane, tight junction.

FUNCTION

Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

CITATIONS

  • Effects of DON on Mitochondrial Function, Endoplasmic Reticulum Stress, and Endoplasmic Reticulum Mitochondria Contact Sites in the Jejunum of Piglets

  • AMPK-PINK1/Parkin Mediated Mitophagy Is Necessary for Alleviating Oxidative Stress-Induced Intestinal Epithelial Barrier Damage and Mitochondrial Energy Metabolism Dysfunction in IPEC-J2

  • Lactobacillus plantarum LLY-606 supplementation ameliorates hyperuricemia via modulating intestinal homeostasis and relieving inflammation.

  • Ma Congcong,et al

    Anti-glioma effect of paclitaxel mediated by specific mode electroacupuncture stimulation and the related role of the Hedgehog pathway

  • Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation

  • Sun Mengzhen,et al

    Sodium butyrate administration improves intestinal development of suckling lambs

  • Wei Weipeng,et al

    Epimedium flavonoids improve cerebral white matter lesions by inhibiting neuroinflammation and activating neurotrophic factor signal pathways in spontaneously hypertensive rats

  • Hong, Y., Zhao, J., Chen, Y. R., Huang, Z. H., Hou, L. D., Shen, B., & Xin, Y.

    Spinal anesthesia alleviates dextran sodium sulfate-induced colitis by modulating the gut microbiota

  • Aixin Hu , Wenxia Huang , Xin Shu , Shiyue Ma , Caimei Yang , Ruiqiang Zhang , Xiao Xiao , Yanping Wu

    Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice

  • Sauchinone alleviates dextran sulfate sodium-induced ulcerative colitis via NAD(P)H dehydrogenase [quinone] 1/NF-kB pathway and gut microbiota

  • Fan Shijie,et al

    Selenium maintains intestinal epithelial cells to activate M2 macrophages against deoxynivalenol injury

  • Lacticaseibacillus rhamnosus TR08 alleviated intestinal injury and modulated microbiota dysbiosis in septic mice

  • Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function

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