CXCL12/SDF1 Recombinant Rabbit Monoclonal Antibody [JJ09-20]
Catalog# ET1701-77
CXCL12/SDF1 Recombinant Rabbit Monoclonal Antibody [JJ09-20]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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unconjugated
Overview
Product Name
CXCL12/SDF1 Recombinant Rabbit Monoclonal Antibody [JJ09-20]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length protein of human SDF1.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 11 kDa
Positive Control
Human kidney tissue.
Conjugation
unconjugated
Clone Number
JJ09-20
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:200
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IHC-P
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1:200
Target
Function
The stromal cell derived factor 1 (SDF1, CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors. The SDF1 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells. SDF1 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells. Many cancer cells express CXCR4, suggesting that SDF1 plays a role in cancer metastasis. Alternative splicing and differential processing during maturation produce a pair of SDF1 isoforms (SDF1α and SDF1β) that have different properties and biological activities. Additional isoforms of SDF1 have been reported.
Background References
1. Shi W et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016).
2. Tanios F et al. CXCR4: A Potential Marker for Inflammatory Activity in Abdominal Aortic Aneurysm Wall. Eur J Vasc Endovasc Surg 50:745-53 (2015).
Sequence Similarity
Belongs to the intercrine alpha (chemokine CxC) family.
Tissue Specificity
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Post-translational Modification
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Subcellular Location
Secreted.
UNIPROT
Synonyms
12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
AI174028 antibody
C-X-C motif chemokine 12 antibody
Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
Chemokine (C-X-C motif) ligand 12 antibody
Chemokine CXC motif ligand 12 antibody
cxcl12 antibody
hIRH antibody
hSDF-1 antibody
Intercrine reduced in hepatomas antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CXCL12/SDF1 antibody (ET1701-77) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-77) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CXCL12/SDF1 with Rabbit anti-CXCL12/SDF1 antibody (ET1701-77) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1701-77, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CXCL12/SDF1 Rabbit Polyclonal Antibody
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
