ZNRF2 Recombinant Rabbit Monoclonal Antibody [JJ0924]
Catalog# ET1701-78
ZNRF2 Recombinant Rabbit Monoclonal Antibody [JJ0924]
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WB
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IHC-P
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Mouse
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Human
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
ZNRF2 Recombinant Rabbit Monoclonal Antibody [JJ0924]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ZNRF2 aa 162-206 / 242.
Species Reactivity
Mouse (Predicted: Human, Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 24 kDa
Positive Control
Mouse testis tissue.
Conjugation
unconjugated
Clone Number
JJ0924
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,500
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IHC-P
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1:50-1:200
Target
Function
May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
Background References
1. Hoxhaj G., et al. 2012. ZNRF2 is released from membranes by growth factors and, together with ZNRF1, regulates the Na+/K+ATPase. J. Cell. Sci. 125:4662-4675.
2. Bian Y., et al. 2014. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics 96:253-262.
Tissue Specificity
Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
Subcellular Location
Cell junction, Cell membrane, Cell projection, Endosome, Lysosome, Membrane, Synapse.
Synonyms
E3 ubiquitin-protein ligase ZNRF2 antibody
Protein Ells2 antibody
RING finger protein 202 antibody
RNF202 antibody
Zinc/RING finger protein 2 antibody
Znrf2 antibody
ZNRF2_HUMAN antibody
Images
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ZNRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"