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ABL2 Recombinant Rabbit Monoclonal Antibody [JF0951]

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Specification

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Catalog# ET1702-07

ABL2 Recombinant Rabbit Monoclonal Antibody [JF0951]

Application

  • WB

  • FC

Reactivity

  • Human

  • Rat

Predicted reactivity

  • Mouse

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ABL2 Recombinant Rabbit Monoclonal Antibody [JF0951]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ABL2 aa 502-552 / 1182.

Species Reactivity

Human, Rat (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 128 kDa

Positive Control

Hela cell lysate, PC-12 cell lysate, Jurkat cell lysate, Raji.

Conjugation

unconjugated

Clone Number

JF0951

RRID

AB_3068595

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

Target

Function

The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including chronic myelocytic leukemia (CML), in which it undergoes a (9;22) chromosomal translocation and produces the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein tyrosine kinase. The related protein tyrosine kinase Arg, also designated Abl2, contains an SH2 and an SH3 domain. Arg has been shown to interact with and to phosphorylate c-Crk.

Background References

1. Klein LE et al. A humanin analog decreases oxidative stress and preserves mitochondrial integrity in cardiac myoblasts. Biochem Biophys Res Commun 440:197-203 (2013).

2. Hutterer C et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013).

Sequence Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.

Tissue Specificity

Widely expressed.

Post-translational Modification

Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).; Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.

Subcellular Location

Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: P42684 Human

SwissProt: Q4JIM5 Mouse

Unigene: 69847 Rat

Synonyms

Abelson murine leukemia viral oncogene homolog 2 antibody

Abelson related gene protein antibody

Abelson tyrosine-protein kinase 2 antibody

Abelson-related gene protein antibody

ABL2 antibody

ABL2_HUMAN antibody

ABLL antibody

ARG antibody

Tyrosine kinase ARG antibody

Tyrosine protein kinase ABL2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ABL2 on different lysates with Rabbit anti-ABL2 antibody (<a href="/products/ET1702-07" style="font-weight: bold;text-decoration: underline;">ET1702-07</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-ABL2 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 128 kDa<br />Observed band size: 128 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-07" style="font-weight: bold;text-decoration: underline;">ET1702-07</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ABL2 on different lysates with Rabbit anti-ABL2 antibody (ET1702-07) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-ABL2 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 128 kDa
    Observed band size: 128 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-07) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ABL2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-07" style="font-weight: bold;text-decoration: underline;">ET1702-07</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Hela cell lysate<br />Lane 2: PC-12 cell lysate<br />Lane 3: Jurkat cell lysate<br /><br />Predicted band size: 128 kDa<br />Observed band size: 128 kDa

    Western blot analysis of ABL2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: PC-12 cell lysate
    Lane 3: Jurkat cell lysate

    Predicted band size: 128 kDa
    Observed band size: 128 kDa

  • Flow cytometric analysis of ABL2 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1702-07" style="font-weight: bold;text-decoration: underline;">ET1702-07</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of ABL2 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-07, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation