Skip to content

Cardiac Troponin I Recombinant Rabbit Monoclonal Antibody [JF0550]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1702-37

Cardiac Troponin I Recombinant Rabbit Monoclonal Antibody [JF0550]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cardiac Troponin I Recombinant Rabbit Monoclonal Antibody [JF0550]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cardiac Troponin I aa 1-47 / 210.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 24 kDa

Positive Control

Rat heart tissue lysate, human heart tissue, HepG2.

Conjugation

unconjugated

Clone Number

JF0550

RRID

AB_3070299

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:200

  • IHC-P

  • 1:1,000

Target

Function

Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer composed of two heavy chains (MHC) and four light chains (MLC); it interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin comprises at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites while slow/cardiac Troponin C has a single calcium binding site.

Background References

1. Wang Y et al. The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits. Mol Med Rep 14:234-42 (2016).

2. Uzun AU et al. Ca(2+)-Currents in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Effects of Two Different Culture Conditions. Front Pharmacol 7:300 (2016).

Sequence Similarity

Belongs to the troponin I family.

Post-translational Modification

Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

Subcellular Location

Cytosol.

UNIPROT

SwissProt: P19429 Human

SwissProt: P23693 Rat

Synonyms

cardiac muscle antibody

Cardiac troponin I antibody

cardiomyopathy, dilated 2A (autosomal recessive) antibody

Cardiomyopathy, familial hypertrophic, 7, included antibody

CMD1FF antibody

CMD2A antibody

CMH7 antibody

cTnI antibody

Familial hypertrophic cardiomyopathy 7 antibody

MGC116817 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Cardiac Troponin I on different lysates with Rabbit anti-Cardiac Troponin I antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>) at 1/2,000 dilution.<br /><br />Lane 1: Rat heart tissue lysate<br />Lane 2: Rat skeletal muscle tissue lysate (negative)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 24 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Cardiac Troponin I on different lysates with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/2,000 dilution.

    Lane 1: Rat heart tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate (negative)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 24 kDa
    Observed band size: 30 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-37) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Cardiac Troponin I antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin I with Rabbit anti-Cardiac Troponin I antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin I with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-37, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • ICC staining of Cardiac Troponin I in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1702-37" style="font-weight: bold;text-decoration: underline;">ET1702-37</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Cardiac Troponin I in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation