TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024]
Catalog# ET1702-56
TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024]
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WB
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IHC-P
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IP
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TGN46 aa 43-79 / 437L5L562:L576.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IP, IF-Cell, FC
Molecular Weight
Predicted band size: 46 kDa
Positive Control
A549 cell lysate, HepG2 cell lysate, HeLa cell lysate, human liver tissue, human brain tissue, HepG2.
Conjugation
unconjugated
Clone Number
JF1-024
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IP
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1:50-1:100
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IHC-P
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1:100-1:1,000
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IF-Cell
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1:100-1:200
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FC
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1:1000
Target
Function
TGN38 (trans-Golgi network protein 2) is a type I integral membrane protein that constitutively cycles between the TGN and plasma membrane where it partitions nascent proteins into carrier vesicles for transport to appropriate destinations in the cell. The cytosolic domain of TGN38 interacts with AP2 Clathrin adaptor complexes via the tyrosine-containing motif (SDYQRL) to direct internalization from the plasma membrane. N- and O-linked oligosaccharide chains attach to the core TGN38 protein to produce a protein present in brain, lung and kidney.
Background References
1. Vural A et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol 196:846-56 (2016).
2. Oetjen S et al. Revisiting the neuronal localization and trafficking of CLN3 in juvenile neuronal ceroid lipofuscinosis. J Neurochem 139:456-470 (2016).
Tissue Specificity
Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Subcellular Location
Cell membrane, Golgi apparatus.
Synonyms
TGN 46 antibody
TGN 51 antibody
TGN38 antibody
TGN38 homolog antibody
TGN46 antibody
TGN48 antibody
TGN51 antibody
TGOLN2 antibody
TGON2_HUMAN antibody
Trans Golgi network integral membrane protein 2 antibody
ExpandImages
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Western blot analysis of TGN46 on different lysates with Rabbit anti-TGN46 antibody (ET1702-56) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 75-100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-56) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TGN46 antibody (ET1702-56) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HepG2 cells labeling TGN46 with Rabbit anti-TGN46 antibody (ET1702-56) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TGN46 antibody (ET1702-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HepG2 cells labeling TGN46.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-56, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Application: IF-cell
Species: Human
Sample: HeLa, U2OS, RPE1
Antibody concentration: 1: 200 (red letter)
Date by conrtesy of: Dr. Yongjia Wang, College of Biological Sciences, China agricultural University
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"